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Maspin gene in-situ hybridization detection kit and detection method and use thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve the problem that human myoepithelial cells no longer have the ability to inhibit the infiltration of breast cancer cells in vitro, and achieve the effect of radically curing the malignant disease of breast cancer, convenient operation and strong specificity

Inactive Publication Date: 2011-03-30
SUZHOU FUYING GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Maspin gene is expressed in normal human breast epithelial cells, but not in most breast cancer cells, and Maspin gene plays an important role in the process of breast cancer. Domestic research by Shao Zhimin and others has shown that mammary gland epithelial cells secrete a large amount of Maspin protein, which can remove Human myoepithelial cells of this protein no longer have the ability to inhibit the invasion of breast cancer cells in vitro

Method used

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  • Maspin gene in-situ hybridization detection kit and detection method and use thereof
  • Maspin gene in-situ hybridization detection kit and detection method and use thereof
  • Maspin gene in-situ hybridization detection kit and detection method and use thereof

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Embodiment 1

[0044] An in situ hybridization detection kit for Maspin gene, comprising a hybridization probe, a marker and a synergist, wherein the sequence of the hybridization probe is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0045] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0046] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0047] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0048] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0049] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0050] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0051] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0052]Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0053] Chr...

Embodiment 2

[0095] A kind of Maspin gene in situ hybridization detection method and its kit application

[0096] 1. Specimen processing

[0097] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0098] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0099] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0100] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with condi...

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Abstract

The invention relates to a Maspin gene in-situ hybridization detection kit, which comprises a hybridization probe and markers, wherein the sequence of the hybridization probe is represented by the sequence SEQ ID No.1. The invention also provides a Maspin gene in-situ hybridization detection method and the use of the kit in the preparation of medicaments for detecting breast cancer. The invention has the advantage that the kit provided by the invention has the characteristics of high sensitivity and high specificity. The detection method of the invention is convenient and simple for operation and can be widely used and promoted in district or above hospitals.

Description

【Technical field】 [0001] The present invention relates to a kit, in particular to a Maspin gene in situ hybridization detection kit and its detection method and application 【Background technique】 [0002] Breast cancer is one of the most common malignant tumors in women. According to statistics, the incidence rate accounts for 7-10% of various malignant tumors in the whole body, and it is second only to uterine cancer in women. Its incidence is often related to heredity, and between the ages of 40 and 60, the incidence rate of women before and after menopause is higher. Only about 1-2% of breast cancer patients are men. Malignant tumors usually occurring in the glandular epithelium of the breast. It is one of the most common malignant tumors that seriously affects women's physical and mental health and even threatens their lives. Breast cancer is a malignant tumor that seriously endangers women's health. the third. According to worldwide statistics, more than 1.2 million...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张云福裘建英
Owner SUZHOU FUYING GENE TECH
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