Probe for detecting substance in body and system for detecting substance in body making use of the probe

A technology for detecting objects and substances, which is applied in the field of systems for detecting substances in the body and the field of instruments for detecting substances in the gingival crevicular fluid, which can solve the problems of cumbersome sample processing, inability to measure the level of substances in the body in real time, time-consuming and labor-intensive, etc.

Inactive Publication Date: 2011-04-27
SONY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] However, because these techniques rely on methods of collecting body fluids such as gingival crevicular fluid using the aforementioned capillary devices or collection instruments and subsequently measuring the levels of substances in the body in the collected body fluids, they require cumbersome sample handling, are time-consuming and labor-intensive, and require skilled operate
Additionally, for continuous assays, it is necessary to perform frequent sampling
In fact, the above techniques cannot measure the level of the substance in the body in real time

Method used

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  • Probe for detecting substance in body and system for detecting substance in body making use of the probe
  • Probe for detecting substance in body and system for detecting substance in body making use of the probe
  • Probe for detecting substance in body and system for detecting substance in body making use of the probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0273] The preparation of the sensing portion usable in the probe for detecting substances in the body according to the present invention was actually carried out. As examples of substances to be incorporated in the sensing section, glucose oxidase, peroxidase, and 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) were used in this embodiment. The sensing part of this embodiment adopts a form in which a detection substance is immobilized in a sensing film. The manufacturing process of the induction part in this embodiment is performed as will be described below.

[0274] 1. Prepare 10 mM ADHP dissolved in dimethylsulfoxide (DMSO).

[0275] 2. Prepare 10 U / mL horseradish peroxidase (POD) dissolved in pure water.

[0276] 3. Aliquots (100 μL) of 10 mM ADHP prepared in step 1 and aliquots (200 μL) of 10 U / mL POD prepared in step 2 were provided separately and after being mixed together, The resulting mixture was diluted with pure water (4.7 mL).

[0277] 4. Prepare 10 U / mL of glucose...

Embodiment 2

[0285] In this embodiment, a physical strength test is performed on the induction part prepared in embodiment 1. The subsequent experimental processing is as follows.

[0286] 1. Provide the slide glass prepared in Example 1 and carrying the sensing portion formed thereon.

[0287] 2. Observe the surface of the slide under a stereomicroscope.

[0288] 3. Provide a root membrane probe ("PDT Sensor Probe Type U.S.", trade name, product of Williame).

[0289] 4. While observing the surface under a stereomicroscope, rub the surface of the sensing portion on the slide glass to apply a load of 20 g.

[0290] 5. Observe for any flaking on the surface of the sensing part by naked eyes and under a stereomicroscope.

[0291] As a result of observation, it was found that the surface of the sensing portion on the glass slide was not peeled off, proving that it exhibited sufficient strength even when it was used in a probe for detecting substances in the body.

Embodiment 3

[0293] This example was performed to confirm that the sensing portion on the glass slide prepared in Example 1 was feasible for performing optical measurements.

[0294] For optical measurement of the sensing part, the following fluorometer system was used. Specifically, the following two types of systems were used for the fluorescence measurement of resorufin produced from ADHP.

[0295] Used as one of the systems is an excitation light source ("LE-1xxx", trade name, product of WT&T Co., Ltd.) incorporating an optical fiber, a stereo microscope ("MCPD-7700", trade name, Otsuka Electronics Co., Ltd. .), a box with a built-in excitation filter, a box with a built-in fluorescence filter, and a fiber optic detector consisting of bound excitation light fibers, fluorescence fibers, and a condenser lens set at the tip. Needle combination. A light source, a box with a built-in excitation light filter, a stereo microscope, and a box with a built-in fluorescence filter are connected ...

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Abstract

Disclosed is a probe for detecting a substance in the body for detecting a substance in the body in gingival crevicular fluid. The probe includes a gingival sulcus insert section and a sensitive part. The gingival sulcus insert section can be inserted into a gingival sulcus. The sensitive part is arranged on the gingival sulcus insert section and contains a detection substance for permitting optical detection of the substance in the body. A system for detecting a substance in the body is also disclosed. The system for detecting a substance in the body includes the probe for detecting a substance in the body, a light irradiator for irradiating light onto the sensitive part in the probe, and a photodetector for detecting optical information from an inside of the sensitive part upon irradiation of light by the light irradiator.

Description

[0001] References to related applications [0002] The present invention contains subject matter of Japanese Patent Application No. 2009-229567 filed with Japan Patent Office on October 1, 2009, subject matter of Japanese Patent Application No. 2009-279758 filed with Japan Patent Office on December 9, 2009, 2010 Subject matter of Japanese Patent Application No. 2010-087060 filed with the Japan Patent Office on April 5 and Japanese Patent Application No. 2010-153058 filed with the Japan Patent Office on July 5, 2010, the entire contents of which are hereby incorporated by reference . technical field [0003] The invention relates to a probe for detecting substances in the body, which can be used as a device for detecting substances in the gingival crevicular fluid. More specifically, the present invention relates to a probe for detecting a substance in the body, and also to a system for detecting a substance in the body using the probe for detecting a substance in the body, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00
CPCA61B5/14532A61B5/682A61B5/1455A61B5/0088
Inventor 大西通博
Owner SONY CORP
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