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Ginsenoside Rh2 extract and preparation method thereof

A technology of ginsenoside and extract, which is applied in the field of ginsenoside Rh2 extract and its preparation, can solve the problems of poor separation of ginsenoside Rh2, and achieve the effects of high yield, high curative effect and convenient operation

Inactive Publication Date: 2013-10-02
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The effect of prior art separation of ginsenoside Rh2 is not good, and the present inventor adopts a new separation technique to test a group of new ginsenoside Rh2 extract and its preparation method in the process of researching ginsenoside Rh2 extract. The ginsenoside Rh2 extract has high curative effect, high purity, good absorption, stable quality, and the preparation method has good separation effect, high content, simple process, convenient operation, low cost, and is suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 ginsenoside Rh2 extract

[0037] Step 1. American ginseng leaves are extracted with water twice, each time for 2 hours, adding 10 times of water each time, the extract is passed through a macroporous adsorption resin column, and firstly eluted with water until the effluent is nearly colorless, followed by 85% ethanol For elution, collect 85% ethanol eluate, concentrate to dryness to obtain total saponins;

[0038] Step 2. Dissolve the total saponins in 20 times the hydrochloric acid solution with a pH of about 1.0, and stir and react at 40°C for 4 hours. After the reaction is completed, adjust the pH to near neutral, precipitate out, collect the precipitate, and obtain the crude product of total ginseng glycosides;

[0039] Step 3. The crude product of total ginseng glycosides is dissolved by heating with ethanol, mixed into 2 times the amount of reversed-phase silica gel, and subjected to reversed-phase silica gel column chromatography, ...

Embodiment 2

[0040] The preparation of embodiment 2 ginsenoside Rh2 extract

[0041]Step 1. American ginseng was extracted with water for 3 times, each time for 1 hour, adding 10 times of water each time, the extract was passed through the macroporous adsorption resin column, firstly eluted with water until the effluent was nearly colorless, and then washed with 30% ethanol Take off, collect the ethanol eluate, concentrate to dryness to obtain total saponins;

[0042] Step 2. Dissolve the total saponins in 20 times the hydrochloric acid solution with a pH of about 1.0, and stir and react at 40°C for 4 hours. After the reaction is completed, adjust the pH to near neutral, precipitate out, collect the precipitate, and obtain the crude product of total ginseng glycosides; Step 3. The crude product of total ginseng glycosides is dissolved by heating with ethanol, mixed into 2 times the amount of reversed-phase silica gel, and subjected to reversed-phase silica gel column chromatography, eluted...

Embodiment 3

[0043] The preparation of embodiment 3 ginsenoside Rh2 extract

[0044] Step 1. Extract Panax notoginseng leaves twice with water, each time for 2 hours, add 10 times the amount of water each time, the extract passes through the macroporous adsorption resin column, and first elutes with water until the effluent is nearly colorless, then use 95% Eluted with ethanol, collected 95% ethanol eluate, concentrated to dryness to obtain total saponins;

[0045] Step 2. Dissolve the total saponins in 20 times the hydrochloric acid solution with a pH of about 1.0, and stir and react at 40°C for 4 hours. After the reaction is completed, adjust the pH to near neutral, precipitate out, collect the precipitate, and obtain the crude product of total ginseng glycosides;

[0046] Step 3: Heat the crude product of total ginseng glycosides with ethanol to dissolve, mix into 2 times the amount of reversed-phase silica gel, perform reversed-phase silica gel column chromatography, elute with 50% ace...

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Abstract

The invention relates to traditional Chinese medicine extract and preparation thereof, in particular to ginsenoside Rh2 extract and preparation thereof. The preparation method comprises the following steps of: 1, extracting a medicinal material which contains ginsenoside components by using water, performing column chromatography on extract liquid through a macroporous adsorption resin column, eluting by using ethanol, collecting eluent, and concentrating the eluent until the eluent is dried so as to obtain total saponins; 2, dissolving the total saponins obtained in the step 1 in acid solution for reaction, adjusting pH to be neutral after the reaction is completed, and collecting precipitate; and 3, dissolving the precipitate in the ethanol, mixing into reverse phase silica gel, performing reverse phase silica gel column chromatography, eluting by using acetonitrile and water mixed liquid, collecting a fraction which is rich in the ginsenoside Rh2, and concentrating the fraction to obtain the ginsenoside Rh2 extract. The prepared ginsenoside Rh2 extract has higher treatment effect and higher purity than that prepared by the prior art.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and its preparation, in particular to a ginsenoside Rh2 extract and its preparation. Background technique [0002] Ginseng has many reports in the treatment of cardiovascular and cerebrovascular diseases. [0003] Ginseng glycosides can treat coronary heart disease and ischemic stroke. [0004] Ginsenoside Rh2 is a secondary saponin produced by hydrolysis of ginsenosides. Pharmacodynamic studies have shown that ginsenoside Rh2 has a significant and specific inhibitory effect on the proliferation of cancer cells such as liver cancer, melanoma B16, cervical cancer and sarcoma S180. Its mechanism of action is as follows: in the process of cancer cell proliferation, through the action of ginsenoside Rh2, the cancer cells stop at a certain proliferation stage and promote their reversion to normal cells. [0005] The effect of prior art separation of ginsenoside Rh2 is not good, and the presen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/258A61K31/704A61P35/00A61K125/00A61K127/00
Inventor 张兰兰宋兆辉蔡楠林万里黄芝娟周水平朱永宏
Owner TIANJIN TASLY PHARMA CO LTD
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