Ginsenoside Rh2 extract and preparation method thereof

Abstract

The invention relates to traditional Chinese medicine extract and preparation thereof, in particular to ginsenoside Rh2 extract and preparation thereof. The preparation method comprises the following steps of: 1, extracting a medicinal material which contains ginsenoside components by using water, performing column chromatography on extract liquid through a macroporous adsorption resin column, eluting by using ethanol, collecting eluent, and concentrating the eluent until the eluent is dried so as to obtain total saponins; 2, dissolving the total saponins obtained in the step 1 in acid solution for reaction, adjusting pH to be neutral after the reaction is completed, and collecting precipitate; and 3, dissolving the precipitate in the ethanol, mixing into reverse phase silica gel, performing reverse phase silica gel column chromatography, eluting by using acetonitrile and water mixed liquid, collecting a fraction which is rich in the ginsenoside Rh2, and concentrating the fraction to obtain the ginsenoside Rh2 extract. The prepared ginsenoside Rh2 extract has higher treatment effect and higher purity than that prepared by the prior art.

Description

technical field

[0001] The invention relates to a traditional Chinese medicine extract and its preparation, in particular to a ginsenoside Rh2 extract and its preparation. Background technique

[0002] Ginseng has many reports in the treatment of cardiovascular and cerebrovascular diseases.

[0003] Ginseng glycosides can treat coronary heart disease and ischemic stroke.

[0004] Ginsenoside Rh2 is a secondary saponin produced by hydrolysis of ginsenosides. Pharmacodynamic studies have shown that ginsenoside Rh2 has a significant and specific inhibitory effect on the proliferation of cancer cells such as liver cancer, melanoma B16, cervical cancer and sarcoma S180. Its mechanism of action is as follows: in the process of cancer cell proliferation, through the action of ginsenoside Rh2, the cancer cells stop at a certain proliferation stage and promote their reversion to normal cells.

[0005] The effect of prior art separation of ginsenoside Rh2 is not good, and the presen...