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Effective fractions of cicer arietinum linn bean sprout and preparation method and application thereof

A technology for effective parts and chickpeas, which is applied to the effective parts of chickpea sprouts and the fields of their preparation and application, and can solve the problems of less research on chickpea sprouts and the like.

Active Publication Date: 2011-05-25
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But relatively little research has been done on the efficacy of chickpea sprouts on tumors

Method used

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  • Effective fractions of cicer arietinum linn bean sprout and preparation method and application thereof
  • Effective fractions of cicer arietinum linn bean sprout and preparation method and application thereof
  • Effective fractions of cicer arietinum linn bean sprout and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 (alcoholic extract)

[0045] Germinate chickpeas in the usual way;

[0046] Wash the selected chickpea raw materials and put them into the container. The container is required to have a rinsing device. The bottom edge should be left with a water hole and a vent hole with a diameter of 5cm, and then add water for soaking to facilitate its operation. Germination, usually soaking for 2 hours, can ensure that the raw beans can absorb enough water. The raw beans that have absorbed enough water after soaking are sprayed with water every 5 hours at room temperature, and the bean sprouts can grow to 6cm long, and the longest does not exceed 10cm;

[0047] Preparation of chickpea sprout alcohol extract:

[0048] Sieve the dried chickpea sprouts powder with 25 mesh, take 5 grams and put it in a round-bottomed flask, add 2 times the volume of methanol with a concentration of 10%, extract 15 times with ultrasonic waves, 1 hour each time, filter with filter paper, and co...

Embodiment 2

[0052] Example 2 (water extract)

[0053] Germinate chickpeas in the usual way;

[0054] Wash the selected chickpea raw materials and put them into the container. The container is required to have a rinsing device, and the bottom edge should be left with a water hole and a vent hole with a diameter of 6 cm, and then add water for soaking, so as to facilitate its Germination, usually soaking for 3 hours, can ensure that the raw beans can absorb enough water. The raw beans that have absorbed enough water after soaking are sprayed with water every 6 hours at room temperature, and the bean sprouts can grow to 7cm long, and the longest is not more than 10cm;

[0055] Preparation of chickpea sprouts water extract:

[0056] Sieve the dried chickpea powder and bean sprout powder with 25 meshes, take 5 grams and place it in a round-bottomed flask, add 2 times the volume of purified water, extract 15 times with ultrasonic waves, 2 hours each time, and filter with gauze and filter pape...

Embodiment 3

[0060] Example 3 (alcoholic extract)

[0061] Germinate chickpeas in the usual way;

[0062] Wash the selected chickpea raw materials and put them into the container. The container is required to have a rinsing device. The bottom edge should be left with a water hole and a vent hole with a diameter of 5cm, and then add water for soaking to facilitate its operation. Germination, usually soaking for 3 hours, can ensure that the raw beans can absorb enough water. The raw beans that have absorbed enough water after soaking are drenched with water every 6 hours at room temperature. The bean sprouts can grow to 6cm long, and the longest does not exceed 10cm;

[0063] Preparation of chickpea sprout alcohol extract:

[0064] Sieve the dried chickpea sprouts powder with 25 mesh, take 5 grams and place it in a round-bottomed flask, add 5 times the volume concentration of 40% ethanol, take 10 times with ultrasonic waves for 3 hours each time, filter with filter paper, and combine the f...

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PUM

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Abstract

The invention relates to effective fractions of cicer arietinum linn bean sprouts and a preparation method and application thereof. The effective fractions of the cicer arietinum linn bean sprouts, namely total saponin and total isoflavone are obtained by a plant chemical extraction separation method and a macroporous resin enrichment process. A bioactivity screening result indicates that the effective fractions of the cicer arietinum linn bean sprouts, namely the total saponin and the total isoflavone can suppress the growth of human adenocarcinoma colon strain Caco-2 cells by inducing cell apoptosis and are used for effectively inducing human adenocarcinoma colon cell apoptosis. A new thought for the research and development of the application of the effective fractions to the preparation of a medicament or a health-care product for treating colon cancer is provided.

Description

technical field [0001] The invention relates to an effective part of chickpea sprouts and a preparation method and application thereof. The effective part is an effective part extracted from chickpea sprouts or dried chickpea sprouts powder, and the effective part is used for the preparation of cancer chemoprevention , especially in the prevention of human colon adenocarcinoma. Background technique [0002] Colon cancer is one of the leading causes of cancer death in the world, and its occurrence and development are closely related to age, family history and dietary structure. With the aging of the population, changes in living environment and dietary structure, the incidence of colon cancer is increasing year by year. Colon cancer has the characteristics of rapid development, short course of disease and high lethality, which makes it an urgent issue to find drugs that can prevent and treat the occurrence and development of colon cancer. Studies have shown that high intake...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A23L1/29A61P35/00A61K131/00A23L33/00
Inventor 阿吉艾克拜尔·艾萨阿布力米提·伊力程珍毛人杰窦君
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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