Dual SYBR Green I real-time fluorescence PCR detection primer and method for porcine parvovirus and porcine circovirus type 2
A porcine circovirus and parvovirus technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fluorescence/phosphorescence, etc., can solve the problems of inability to quantify, false negative, spread, etc., and achieve good specificity
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[0026] The sequences of porcine parvovirus and porcine circovirus type 2 dual SYBR Green I real-time fluorescent PCR detection primers are as follows:
[0027] The sequence of the porcine circovirus type 2 primer is as follows:
[0028] Upstream primer P1: 5′-TAA CTA CTC CTC CCG CCA TAC-3′;
[0029] Downstream primer P2: 5′-GCC TAC GTG GTC TAC ATT TCC-3′;
[0030] The sequences of porcine parvovirus primers are as follows:
[0031] Upstream primer P3: 5′-TGG GAG GGC TTG GTT AGA-3′;
[0032] Downstream primer P4: 5'-TGG TGG TGA GGT TGC TGA T-3'.
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