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Human miR (microRNA)-1233 antisensenucleic acid and application thereof

An antisense and oligonucleotide technology, applied in the field of biomedical materials, can solve the problems of poor curative effect of patients with distant metastasis, poor curative effect, and low survival rate of patients with middle and advanced stages, and achieve inhibition of growth and malignant proliferation effect of ability

Active Publication Date: 2013-04-10
SUZHOU GENEPHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the past 30 years, although the comprehensive treatment of tumors has been very common clinically, the comprehensive treatment based on surgery and supplemented by radiotherapy and chemotherapy has not significantly improved the survival rate of cancer patients, and the 5-year overall survival rate is still low, hovering At about 30% to 55%, there is no significant improvement, and the 5-year survival rate of middle and advanced patients is even lower, about 20%.
Moreover, these methods have their own limitations, especially for the middle-advanced and relapsed patients, and the curative effect is even worse for those with distant metastasis.

Method used

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  • Human miR (microRNA)-1233 antisensenucleic acid and application thereof
  • Human miR (microRNA)-1233 antisensenucleic acid and application thereof
  • Human miR (microRNA)-1233 antisensenucleic acid and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1. Detection of inhibitory activity of miR-1233 antisense oligonucleotides on human glioma cell line U87 / MG

[0033] First, the miR-1233 antisense oligonucleotide was synthesized by Shanghai Jima Pharmaceutical Technology Co., Ltd., the sequence is: 5'-CUGCGGGAGGACAGGGCUCA-3'. All the sequences used in the examples were synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0034] Cell culture:

[0035] U87 / MG cells (purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences), cultured in 10% FBS-DMEM medium (FBS was purchased from Gibco, DMEM was purchased from Hyclone), 37 ° C, 5% CO 2 to cultivate. Collect U87 / MG cells in good growth state, count by centrifugation, and use 2×10 3 Spread each well in a 96-well plate at 37°C, 5% CO 2 Cultivate for 24h.

[0036] Transfection:

[0037] 1) One day before transfection, inoculate cultured cells in a 96-well plate with an appropriate amount of culture m...

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Abstract

The invention discloses antisense oligonucleotide for suppressing the expression of human miR (microRNA)-1233 and application thereof. The antisense oligonucleotide is specifically combined to human miR-1233, and comprises a sequence complementary to at least 13 continuous nucleotide complementary sequences in a nucleotide sequence, i.e., 5'-UGAGCCCUGUCCUCCCGCAG-3', preferably 5'-CUGCGGGAGGACAGGGCUCA-3'. The antisense oligonucleotide disclosed by the invention can be ribonucleotide, deoxyribonucleotide or the chimera of the ribonucleotide and the deoxyribonucleotide, and any ribonucleotide in the chain is modified. By adopting the miR-1233 antisense oligonucleotide disclosed by the invention, the expresssion of the miR-1233 in glioma cells of a human brain can be effectively suppressed, the growth and proliferation of the cells are suppressed, and glioma and other tumors with high miR-1233 expression are effectively treated.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and the field of medicine. Specifically, the present invention relates to a use of microRNAs (miRNA), in particular to human microRNA-1233 (human miR-1233) antisense nucleic acid and its application. The antisense nucleic acid can be complementary to human miR-1233, thereby inhibiting the expression of human miR-1233 to play an anti-tumor effect. The invention also relates to a pharmaceutical composition containing the miRNA antisense nucleic acid. Background technique [0002] miRNAs are small non-coding RNAs, 20-25bp in length, usually transcribed by RNA polymerase II (Pol II), and generally the initial product is a large one with a cap structure (7MGpppG) and a polyA tail (AAAAA) The pri-miRNA. These pri-miRNAs are processed into pre-miRNA precursor products consisting of 70 nucleotides under the action of RNase III Drosha and its cofactor Pasha. RAN-GTP and exportin 5 transpor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00A61P25/00
Inventor 丁侃张佩琢李捷段春晓沈孝坤
Owner SUZHOU GENEPHARMA