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Method for determining content of hematoxylin in brazilwood by molecular imprinting method

A technology of hematoxylin and hematoxylin, which is applied in the field of molecular imprinting to determine the content of hematoxylin in hematoxylin, can solve the problems of high molecular weight of hematoxylin, achieve the effects of increased sensitivity, reduced test error, and enhanced current intensity

Active Publication Date: 2013-09-18
江苏泗水纺织有限公司
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  • Abstract
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Problems solved by technology

At present, molecular imprinting method is mainly used in the detection of small molecules, and hematoxylin has a relatively large molecular weight, and there is no report on its molecular imprinting analysis method.

Method used

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  • Method for determining content of hematoxylin in brazilwood by molecular imprinting method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The hematoxylin is crushed into 60-mesh granules, 10 g of the sample is taken and the extract (water solution containing 5% ethanol by mass fraction) is added to 50 mL, and extracted by ultrasonic treatment for 5 minutes. The frequency of the ultrasonic generator is 20 kHz, and the power is 100 W. The extraction temperature was 20°C. The residue was extracted 3 times again, and the extracts were 50mL, 30mL, and 20mL respectively. The extracts were combined, and the extract was filtered and then adjusted to 200 mL (pH=3) with a buffer solution, ready for electrochemical determination.

[0018] Take hematoxylin standard products of different quality, dissolve them in the buffer solution with pH=3, use the hematoxylin-pyrrole / graphite imprinted electrode as the working electrode, the platinum electrode as the counter electrode, and the calomel electrode as the reference electrode. When the speed is 30mV / s, the cyclic voltammetry curve is measured, the highest peak positio...

Embodiment 2

[0020] The hematoxylin is crushed into 60-mesh granules, 10 g of the sample is taken and the extract (water solution containing 30% ethanol by mass fraction) is added to 50 mL, and extracted by ultrasonic treatment for 30 minutes. The frequency of the ultrasonic generator is 80 kHz, and the power is 400 W. The extraction temperature is 300C. The residue was extracted 3 times again, and the extracts were 50mL, 30mL, and 20mL respectively. The extracts are combined, and the extracts are filtered and then adjusted to 200 mL (pH=9) with a buffer solution, ready for electrochemical determination.

[0021] Hematoxylin standard products of different qualities were taken and dissolved in a buffer solution with pH=7, using hematoxylin-pyrrole / graphite as the working electrode, platinum electrode as the counter electrode, and calomel electrode as the reference electrode. At 90mV / s, the cyclic voltammetry curve was measured, the highest peak position of the oxidation peak was at 0.19V, ...

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Abstract

The invention provides a method for determining content of hematoxylin in brazilwood by a molecular imprinting method. The method is characterized in that a hematoxylin-oyrrole / graphite imprinting electrode is used as a three-electrode system in the working electrode; in a buffer solution in which pH is equal to 3 to 11, under the condition that the temperature is 20 to 80 DEG C and the scanning speed is 30 to 600mv / s, the CV curve in the hematoxylin solution of different concentrations can be measured, thus obtaining a linear response relationship, a lower detection limit, and the like. Compared with other electrodes, the method has the advantages that the current intensity is improved by 2 orders of magnitude, and the measurement sensitivity can be greatly improved.

Description

technical field [0001] The invention relates to a method for determining the content of hematoxylin in hematoxylin by molecular imprinting method. Background technique [0002] The research on the pharmacological effects of Sappan began in the 1970s, and there are many studies at home and abroad. In the past ten years, dozens of reports have shown that Sappan has many clinical pharmacological effects, including immunosuppressive effect, anti-tumor effect, Antibacterial and anti-inflammatory effects, sedative and analgesic effects, hypoglycemic effects, etc. Since hematoxylin is the main component of hematoxylin showing curative effect, the quantitative analysis and research of hematoxylin has extremely important significance. [0003] Hematoxylin belongs to plant polyphenols. Because plant polyphenols coexist and are similar to flavonoids, anthraquinones, simple phenols and lignin in plants. It itself is a kind of mixture with very similar structure and properties, and po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/30
Inventor 高海燕仇小飞侯秀良汪茹刘瑛陈新许芳萍
Owner 江苏泗水纺织有限公司
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