Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing granulocyte-macrophage colony stimulating factor microsphere

A technology of colony-stimulating factor and macrophages, which is applied in pharmaceutical formulas, medical preparations containing active ingredients, peptide/protein components, etc., can solve problems such as inability to overcome the encapsulation rate, achieve regular particles without adhesion, and improve curative effect , the effect of reducing the cost of preservation

Inactive Publication Date: 2013-09-25
SHANGHAI JIAOTONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it still cannot overcome the low encapsulation efficiency and the shortcoming of burst release and incomplete release. So far, there has been no report on the preparation of granulocyte-macrophage colony-stimulating factor microspheres by the glycerol method.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing granulocyte-macrophage colony stimulating factor microsphere
  • Method for preparing granulocyte-macrophage colony stimulating factor microsphere
  • Method for preparing granulocyte-macrophage colony stimulating factor microsphere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: A method for preparing granulocyte-macrophage colony-stimulating factor (GM-CSF) microspheres by the glycerol method

[0026] (1) Take 10 mg of granulocyte-macrophage colony-stimulating factor dextran particles of 5 μm and a dichloromethane solution of polylactic acid-glycolic acid (50:50, PLGA) with a molecular weight of 47,000 at a concentration of 20% by weight Stir, vortex or sonicate 1mL for 1-5 minutes to form a uniform suspension, that is, solid-in-oil (S / O) emulsion;

[0027] (2) Add the emulsion obtained in step (1) dropwise to the glycerin-modified aqueous phase (W) (W) containing 20% ​​by weight and stir, vortex or sonicate for 0.1-5 minutes to form double emulsion;

[0028] (3) The double emulsion of step (2) is added dropwise in the 1000mL sodium chloride solution that the weight percent concentration is 5% (w / w) and stirs 2-4 hour;

[0029] (4) Collect the microspheres obtained in step (3) by centrifugation, wash with water for 3-5 times, and fre...

Embodiment 2

[0032] Embodiment 2: A kind of method for preparing granulocyte-macrophage colony-stimulating factor (GM-CSF) microspheres by glycerol method

[0033] (1) Take 50 mg of 10 μm granulocyte-macrophage colony-stimulating factor dextran particles and 1 mL of a dichloromethane solution of 20% polylactic acid-glycolic acid (50:50, PLGA) with a molecular weight of 47,000 and stir , Vortex or ultrasonic for 1-5 minutes to form a uniform suspension, that is, solid-in-oil (S / O) emulsion;

[0034] (2) Add the emulsion obtained in step (1) dropwise to the glycerin-modified aqueous phase (W) (W) containing a concentration of 40% by weight and stir, vortex or sonicate for 0.1-5 minutes to form a double emulsion;

[0035] (3) The double emulsion of step (2) is added dropwise in the 1000mL sodium chloride solution that the weight percent concentration is 10% (w / w) and stirs 2-4 hour;

[0036] (4) Collect the microspheres obtained in step (3) by centrifugation, wash with water for 3-5 times, a...

Embodiment 3

[0039] Embodiment three: a kind of method for preparing granulocyte-macrophage colony-stimulating factor (GM-CSF) microspheres by glycerol method

[0040] (1) Take 5 mg of granulocyte-macrophage colony-stimulating factor dextran particles of 5 μm and 1 mL of a dichloromethane solution of polylactic acid (PLA) with a molecular weight of 83,000 at a concentration of 20% and stir, vortex or sonicate for 1-5 A uniform suspension is formed within minutes, that is, a solid-in-oil (S / O) emulsion;

[0041] (2) Add the emulsion obtained in step (1) dropwise to the glycerin-modified aqueous phase (W) (W) containing 60% by weight and stir, vortex or sonicate for 0.1-5 minutes to form double emulsion;

[0042] (3) The double emulsion of step (2) is added dropwise in the 1000mL sodium chloride solution that the weight percent concentration is 1% (w / w) and stirs 2-4 hour;

[0043] (4) Collect the microspheres obtained in step (3) by centrifugation, wash with water for 3-5 times, and freeze...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

A method for preparing a granulocyte-macrophage colony stimulating factor microspheres, belonging to the technical field of nano medicaments, comprises the steps of dispersing granulocyte-macrophage colony stimulating factor glucan particles in organic solution of materials with a sustained release or controlled release function to form suspension, then adding the suspension in an oil phase (O), stirring or swirling to form microspheres, transferring the microspheres into an aqueous phase modified by glycerol to form multiple emulsion, and finally carrying out curing on the multiple emulsion to obtain granulocyte-macrophage colony stimulating factor microspheres. The microspheres prepared by the invention have no adhesion, high encapsulation efficiency, small burst release and high drug loading rate.

Description

technical field [0001] The invention relates to a method in the technical field of nanometer medicine, in particular to a method for preparing granulocyte-macrophage colony-stimulating factor (GM-CSF) microspheres by a glycerol method. Background technique [0002] In the pharmaceutical industry, from drug discovery to clinical application, the last link is drug preparation. Some of the drugs need long-term administration to be cured; others need targeted and other local administration. To achieve these goals, raw materials must be prepared into corresponding dosage forms. For example, drugs that require long-term administration but have a short half-life in the body should be prepared as sustained-release dosage forms; for the treatment of some tumors, some drugs need to be targeted to the disease, such as embolization microsphere preparations that target tumor blood vessels; Since recombinant technology has been used in the expression and production of therapeutic protei...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/16A61K38/19A61K47/34A61K47/38
Inventor 郑瑞媛任甜甜洪晓芸袁伟恩冯艳秦明杰
Owner SHANGHAI JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com