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Vegetable oil vaccine adjuvant and preparation method and application thereof

A vaccine adjuvant and vegetable oil technology, applied in the field of vegetable oil vaccine adjuvant and its preparation, can solve the problems of large local irritation reaction at the injection site, high requirements on emulsion conditions, food safety threats, etc. The effect of improving safety

Active Publication Date: 2019-08-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. Oil adjuvants use mineral oil as raw material, which will inevitably result in residues of condensed ring aromatic hydrocarbons in the adjuvant products, which have carcinogenic effects. The use of this adjuvant to make vaccines for immunization of animals may pose a threat to food safety. potential threat;
[0005] 2. Vaccines prepared with traditional mineral oil adjuvants cause large local irritation at the injection site;
[0006] 3. The traditional mineral oil adjuvant needs to be mixed with the antigen solution to form an emulsion under high shear force;
But the weak point that this vegetable oil adjuvant exists is that the condition requirement of preparation emulsion is high, and the adjuvant oil phase and the water phase containing antigen need to form stable emulsion under high shear force (18000rpm)
Moreover, the adjuvant effect is weak

Method used

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  • Vegetable oil vaccine adjuvant and preparation method and application thereof
  • Vegetable oil vaccine adjuvant and preparation method and application thereof
  • Vegetable oil vaccine adjuvant and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the preparation method of vegetable oil vaccine adjuvant, comprises the following steps:

[0054] S1. Preparation of component A:

[0055] 1.1. Take 600ml of rapeseed oil and heat it in an aluminum pot until the oil temperature rises to 20°C; while stirring, add 240ml of Span-80 (product of Shanghai Fuqi Industry and Trade Co., Ltd.) and 40ml of Tween to the rapeseed oil -80 (product of Sinopharm Chemical Reagent Co., Ltd.), completely mix rapeseed oil, Span-80 and Tween-80 to obtain mixture Ⅰ.

[0056] 1.2. Add 44 mg vitamin E (product of Sigma Company) to the mixture I obtained in step 1.1, and obtain mixture II after the vitamin E is fully dissolved;

[0057] 1.3. Add 7.1ml of ginsenoside solution with a mass concentration of 15mg / mL to the mixture II obtained in step 1.2, stir evenly, filter and sterilize (pass through a 0.22 micron filter membrane), and cool the obtained filtrate to room temperature (25°C). Obtain component A;

[0058] The preparat...

Embodiment 2

[0065] Embodiment 2. Change the preparation of component A in step S1 of embodiment 1 to the following steps:

[0066] 1.1. Take 600ml of soybean oil and heat it in an aluminum pot until the temperature of the oil rises to 25°C; while stirring, add 240ml of Span-80 and 50ml of Tween to the soybean oil to make soybean oil, Span-80 and Tween The three are mixed completely to obtain mixture I.

[0067] 1.2. Add 445 mg vitamin E to the mixture I obtained in step 1.1, and obtain the mixture II after the vitamin E is fully dissolved;

[0068] 1.3. Add 5ml of ginsenoside solution with a concentration of 15mg / mL to the mixture II obtained in step 1.2, stir evenly, filter and sterilize, and cool the obtained filtrate to room temperature (25°C) to obtain component A;

[0069] That is, in this embodiment, every 100ml of component A contains about 8.4mg of ginsenoside and 50mg of vitamin E;

[0070] All the other are equal to embodiment 1.

Embodiment 3

[0071] Embodiment 3. Change the preparation of component A in step S1 of embodiment 1 to the following steps:

[0072] 1.1. Take 600ml of sesame oil and heat it in an aluminum pot until the oil temperature rises to 30°C; while stirring, add 240ml of Span-80 and 60ml of Tween-80 to the sesame oil to make the sesame oil, Span-80 and Tween-80 80 The three are mixed completely to obtain mixture I.

[0073] 1.2. Add 900 mg of vitamin E to the mixture I obtained in step 1.1, and obtain mixture II after the vitamin E is fully dissolved;

[0074] 1.3. Add 5.4ml of ginsenoside solution with a concentration of 10mg / mL to the mixture II obtained in step 1.2, stir evenly, filter and sterilize, and cool the obtained filtrate to room temperature (25°C) to obtain component A;

[0075] That is, in this example, every 100ml of component A contains 6mg of ginsenoside and 100mg of vitamin E;

[0076] All the other are equal to embodiment 1.

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Abstract

The invention relates to the technical field of vaccine adjuvants, in particular to a vegetable oil vaccine adjuvant and a preparation method and application thereof. The preparation method of the vegetable oil vaccine adjuvant comprises the steps of preparing a component A, wherein the component A consists of vegetable oil, span-80, tween-80, vitamin E and ginsenoside; preparing a component B, wherein the component B consists of glycerinum and 1,2-propylene glycol; and uniformly mixing the component A with the component B so as to obtain the vegetable oil vaccine adjuvant. The vegetable oil vaccine adjuvant can be used for preparing vaccines.

Description

technical field [0001] The invention relates to the technical field of vaccine adjuvants, in particular to a vegetable oil vaccine adjuvant and its preparation method and application. Background technique [0002] China is the largest animal breeding country in the world, with a large number of livestock and poultry. About 700 million pigs and 11.3 billion broiler chickens are slaughtered every year (1,2). There are many kinds of animal diseases. In large-scale farming, due to the high density of animals, the diseases are easy to spread. Injecting animals with vaccines can improve the resistance of animals to epidemic diseases, which can reduce the occurrence of epidemic diseases and reduce the economic losses caused by epidemic diseases to farmers. It is an important measure to prevent animal epidemics. Take foot-and-mouth disease, an infectious disease of cloven-hoofed pigs, as an example. my country implements a compulsory immunization control policy, and needs to inocul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P37/04
CPCA61K39/39A61K2039/55511A61K2039/55566A61K2039/55588A61P37/04
Inventor 胡松华
Owner ZHEJIANG UNIV
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