Application of total dendrobium crepidatum alkaloids in preparing medicament for treating acute and chronic inflammation, acute and chronic arthritis or rheumatoid arthritis diseases

A technology of total alkaloids and Dendrobium rosea, which can be used in pharmaceutical formulations, organic active ingredients, medical preparations containing active ingredients, etc., can solve the problems of unseen reports on the activity of medicinal materials and their monomer substances, and achieve good anti-inflammatory activity , clear activity and good stability

Active Publication Date: 2013-05-01
CHINA PHARM UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] It is only seen that in the 1950s, foreign scholars had carried out a small amount of research reports on the chemical components of Dendrobium rosea, from which five alkaloid components were isolated and identified (see the figure below Magnus Elander et al, Studies on Orichidaceae Alkaloids, Acta Chenical Scandinavica 27(1973):1907-1913), there is no report on the activity of medicinal materials and their monomer substances

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of total dendrobium crepidatum alkaloids in preparing medicament for treating acute and chronic inflammation, acute and chronic arthritis or rheumatoid arthritis diseases
  • Application of total dendrobium crepidatum alkaloids in preparing medicament for treating acute and chronic inflammation, acute and chronic arthritis or rheumatoid arthritis diseases
  • Application of total dendrobium crepidatum alkaloids in preparing medicament for treating acute and chronic inflammation, acute and chronic arthritis or rheumatoid arthritis diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The crushed and dried Dendrobium rose medicinal material was extracted 3 times with 10 times the amount of ethanol-water mixed solvent (90:10), the extraction time was 3, 3, and 2 hours respectively, filtered, the filtrate was concentrated to dryness, and soaked with 5% hydrochloric acid-water solution, Adjust the pH to weak acidity (pH=4~5), the aqueous solution is super-acid styrene-type cation exchange resin column (model: 001×7), remove impurities with 3 times the amount of 60% ethanol water mixed solvent, and then use 2 times the amount of Elute with ammonia water-ethanol solvent (1:8), adjust the pH value of the eluent to neutral with hydrochloric acid, and concentrate to obtain the total alkaloids of Dendrobium rosea. The content of total alkaloids in Dendrobium rosea was determined by acid dye colorimetry. The determination method is as follows, and the total alkaloid content is 80%.

[0017] test methods

[0018] Standard solution preparation Weigh and dry to...

Embodiment 2

[0024] Method b. The crushed Dendrobium officinale medicinal material was reflux extracted with 10 times the amount of 95% ethanol for 3 times, each extraction time was 3, 3, and 2 hours respectively, and then refluxed with 10 times the amount of 75% ethanol for 2 hours, and reduced pressure Recover the solvent to obtain a concentrated solution, extract once with an equal volume of petroleum ether (60-90°C), discard the organic layer, add 0.3 times the amount of 5% hydrochloric acid, stir and filter, collect the filtrate, and adjust the pH value of the filtrate to 9 with ammonia water ~10 (visible precipitation), extracted 3 times with equal volume of chloroform, and the chloroform extract was concentrated into a reddish-brown extract to obtain the total alkaloids of Dendrobium rosea, and the total alkaloid content was measured according to the method of Example 1, and the total alkaloids were prepared by this method The content is 86%.

Embodiment 3

[0026] Method c. The crushed Dendrobium rose medicinal material was soaked with 8 times the amount of 85% ethanol for 24 hours, percolated, the percolated solution was combined, the ethanol was recovered under reduced pressure and concentrated to obtain a concentrated water extract, which was extracted once with an equal volume of petroleum ether. After extraction, add 5% hydrochloric acid to the aqueous solution to pH 2-3, filter, add saturated sodium carbonate solution to the filtrate to pH 9-10, extract twice with equal volume of chloroform, wash the extract with water, and evaporate to dryness under reduced pressure. Total alkaloids from Dendrobium rosea. The total alkaloid content was measured according to the method in Example 1, and the total alkaloid content prepared by this method was 83%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an application of total dendrobium crepidatum alkaloids in preparing a medicament for treating acute and chronic inflammation, acute and chronic arthritis or rheumatoid arthritis diseases, and the content of the total dendrobium crepidatum alkaloids is 80-91 percent; and shown through a great amount of experiments by an inventor, the total dendrobium crepidatum alkaloids have a good effect on inflammation treatment. The total dendrobium crepidatum alkaloids have better stability and good anti-inflammation activity, and can be used for preparing medicaments for treating corresponding diseases. The total dendrobium crepidatum alkaloids have clear activity, and the experimental materials come from plants, so that the cost is low.

Description

technical field [0001] The present invention relates to the extract of Dendrobium rosea, in particular to the application of total alkaloids of Dendrobium rosea in the preparation of medicines for treating acute and chronic inflammation, acute and chronic arthritis or rheumatoid arthritis. Background technique [0002] It is only seen that in the 1950s, foreign scholars had carried out a small amount of research reports on the chemical components of Dendrobium rosea, from which five alkaloid components were isolated and identified (see the figure below Magnus Elander et al, Studies on Orichidaceae Alkaloids, Acta Chenical Scandinavica 27(1973): 1907-1913), there is no report on the activity of medicinal materials and their monomeric substances. [0003] Contents of the invention [0004] The object of the present invention is: a kind of total organism of the Chinese medicinal material Dendrobium roseum which has the medicinal use for treating acute and chronic inflammat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/407
Inventor 张朝凤张勉戴岳王峥涛许翔鸿赵昕张天竞
Owner CHINA PHARM UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap