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Method for extracting aucubin from eucommia ulmoides oliver seed draff

A technology of aucubin and seed meal, which is applied in the field of medicine, can solve the problems of long production cycle, cumbersome operation, and inapplicability to industrial production, and achieve the effects of small usage, simple operation, and low cost

Inactive Publication Date: 2011-10-19
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is recorded that aucubin was prepared from Eucommia ulmoides seed meal (Peng Mijun, Yin Dazhong, Liu Liping et al. Preparation of aucubin from Eucommia ulmoides seed meal. Fine Chemical Industry, 2007, 24(3):243-247), but Among them, the microwave method and preparative chromatography are used for preparation, so it is only suitable for a small amount of preparation in the laboratory, and the operation is cumbersome and the production cycle is long, so it is not suitable for industrial production.

Method used

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  • Method for extracting aucubin from eucommia ulmoides oliver seed draff
  • Method for extracting aucubin from eucommia ulmoides oliver seed draff

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Take Eucommia ulmoides seed meal and crush it to 10 mesh, take 1 kg and put it in a flash extractor, add 5 L of ethanol solution with a volume concentration of 20%, flash extraction 3 times, 10 minutes each time, stirring speed 10000r / min, extraction start temperature Combine the extracts at 55°C; the extracts are concentrated in a multi-stage flash evaporator to a thick paste and dissolved in water to make 1 L of concentrated liquid, put in a separatory funnel, refrigerate and stand overnight at 4°C, discard the upper suspended matter, The water layer was extracted 3 times with 1 L of petroleum ether, and the extracted water layer was used as the sample solution; the sample solution was absorbed by a 0.5L AB-8 macroporous resin column, washed with water until colorless, and then washed with 2L 90% Eluted with ethanol solution, collected the eluate, concentrated under reduced pressure, and dried at low temperature to obtain 28 g of Eucommia ulmoides seed meal extract, of...

Embodiment 2

[0034] Take Eucommia ulmoides seed meal and crush it to 20 mesh, take 1 kg into a flash extractor, add 40L of 90% ethanol solution, flash extract for 0.2 minutes, once, stir at 12000r / min, start extraction temperature at 0°C, filter , the filtrate was concentrated to a thick paste with a multi-stage flash evaporator, dissolved in water to make 1 L of concentrated solution, placed in a separatory funnel, refrigerated and left overnight at 4°C, the upper layer of suspended matter was discarded, and the water layer was added with 1 L of petroleum ether Extracted once, the extracted water layer was used as the sample solution; after the sample solution was absorbed by a 0.5L NKA-II macroporous resin column, washed with water until colorless, then eluted with 3.5L 20% ethanol solution, collected and washed The liquid was removed, the eluent was concentrated under reduced pressure, and dried at low temperature to obtain 26 g of Eucommia ulmoides seed meal extract, wherein the content...

Embodiment 3

[0036] Take Eucommia ulmoides seed meal and crush it to 15 mesh, take 1kg and put it in a flash extractor, add 20L 60% ethanol solution, flash extract twice, each time for 3 minutes, stirring speed 11000r / min, extraction start temperature is 25°C, Combine the extracts, use a multi-stage flash evaporator to concentrate the extracts to a thick paste, add water to dissolve to make a 1 L concentrate, put it in a separatory funnel, refrigerate and stand overnight at 4°C, discard the upper layer of suspended matter, and use the water layer again 1 L of petroleum ether was extracted twice, and the extracted water layer was used as the sample solution; the sample solution was adsorbed by a 0.5L H103 macroporous resin column, washed with water until colorless, and then eluted with 2.5L 40% ethanol solution. The eluate was collected, concentrated under reduced pressure, and dried at low temperature to obtain 36 g of Eucommia ulmoides seed meal extract, of which the aucubin content was 60...

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Abstract

The invention belongs to the technical field of medicine and in particular relates to a method for extracting aucubin from eucommia ulmoides oliver seed draff. The method comprises the following steps: carrying out flash type extraction or ultrasonic extraction on crude eucommia ulmoides oliver seed draff powder; removing an extraction solvent and water-insoluble substances in extract; based on a water layer extracted by an organic solvent for an aqueous solution as a sampling liquid, processing the sampling liquid by virtue of a macroporous absorption resin column, and successively eluting with water and ethanol; collecting alcohol eluent, then concentrating, and drying so as to obtain the aucubin. According to the invention, the raw material cost is cheap; the method has the advantages of fast extraction speed, short period, low energy consumption and no pollution and is simple to operate; the product is high in yield and stable in quality; and the method is easy for industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for extracting aucubin from Eucommia ulmoides seed dregs. Background technique [0002] Aucubin (aucubin) is an iridoid compound with the molecular formula C 15 h 22 o 9 , Molecular weight is 346.33, mp181℃, easily soluble in water, methanol, soluble in ethanol, almost insoluble in chloroform, ether, benzene and petroleum ether. The compound mainly exists in Eucommia Eucommia ulmoides Oliver, Cornaceae Peachleaf Coral ( Aucubachinensis ), Plantainaceae Plantain ( Plantago ) and other plants. Aucubin has the functions of promoting stem cell regeneration, significantly inhibiting DNA replication of hepatitis B virus, anti-oxidation, anti-inflammation, promoting collagen synthesis, photoprotection, anti-osteoporosis, neuroprotection and inducing apoptosis. And polymers are also high-quality natural antibiotics. The structural formula of aucubin is as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 李钦杜红岩张木升马凤先杜兰英
Owner HENAN UNIVERSITY
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