Cultivating method for cordyceps militaris by using manyprickle acathopanax root

A cultivation method and technology of Acanthopanax senticosus, applied in the fields of botanical equipment and methods, fertilizer mixtures, gardening, etc., can solve the problems of inconvenient medicines and large dosages, and achieve the reduction of taking methods, enhancement of supplementary efficacy, and shortening of growth. effect of cycles

Inactive Publication Date: 2012-07-25
王满玉
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In Chinese medicine, Cordyceps militaris is often used in combination with various tonic Chinese medicines to achieve synergistic effects. However, when patients use traditional Chinese medicines in combination, they often stop using the medicines due to the large dosage and the inconvenience of taking them.

Method used

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  • Cultivating method for cordyceps militaris by using manyprickle acathopanax root
  • Cultivating method for cordyceps militaris by using manyprickle acathopanax root
  • Cultivating method for cordyceps militaris by using manyprickle acathopanax root

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specific Embodiment approach 1

[0011] Specific implementation mode 1: The cultivation method of Acanthopanax senticosus Cordyceps militaris in this embodiment is carried out according to the following steps: 1. Inoculate the Cordyceps militaris strain Yufeng No. 2 on the PDA solid medium and culture it in the dark in a biochemical incubator at 28°C for 5 days , To obtain the activated strain; 2. Cut the activated strain into a 1cm×1cm colony and inoculate it into 300ml liquid seed culture medium. After incubating for 24 hours, it is placed under the condition of 23℃ and 120r / min. Cultivate in the dark for 6 days to obtain a bacterial suspension, and then add 200ml of sterile water to obtain a liquid strain; 3. Inoculate 5ml of the liquid strain into a culture flask containing Acanthopanax senticosus solid medium, and place it at a temperature of 22℃, Incubate in the dark for 10 to 14 days at a humidity of 55% to 60% to obtain mycelium, then move to the light and place at a temperature of 23°C, a humidity of 5...

specific Embodiment approach 2

[0019] Specific embodiment two: this embodiment is different from specific embodiment one in that the PDA solid medium in step one is composed of 200g potato liquid, 20g glucose, 18g agar and 1000ml water, and the pH is natural; Preparation: 200g peeled potatoes, cut into pieces and boiled for 30 minutes, filtered with gauze to make up to 1000ml, pH natural, autoclaved at 121°C for 30 minutes. Other steps and parameters are the same as in the first embodiment.

specific Embodiment approach 3

[0020] Specific embodiment three: This embodiment is different from specific embodiment one in that in step two, each L of liquid seed culture medium consists of 200g potato liquid, 20g glucose, 1g magnesium sulfate, 1g potassium dihydrogen phosphate and the balance of water. Composition, natural pH; preparation of the potato liquid: 200g peeled potatoes, cut into pieces, boiled for 30 minutes, filtered with gauze to make up to 1000ml, pH natural, autoclaved at 121°C for 30 minutes. Other steps and parameters are the same as in the first embodiment.

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Abstract

The invention discloses a cultivating method for cordyceps militaris by using manyprickle acathopanax root and relates to a cultivating method for cordyceps militaris. The method is used for simplifying Chinese medicine compatibility dosage, increasing the utilization rate of cordyceps militaris and reducing the medicine consumption. The method comprises the following steps of: 1, activating strains; 2, preparing liquid strains; and 3, inoculating the liquid strains into a manyprickle acathopanax root solid culture medium for dark cultivation to obtain mycelia, then transferring to light and culturing until sporocarp grows up to 2 cm in height, after gas introduction, continuing culturing until the sporosarp grows up to 4-8 cm in height, yellow powdery substances appear on the surface of the sporosarp as well as small thorns appear on the top end, and harvesting. According to the method disclosed by the invention, active ingredients of the manyprickle acathopanax root are transferred into the sporocarp of cordyceps militaris, so that a beneficial curative effect of cordyceps militaris is indirectly enhanced, the method of compatibility of cordyceps militaris and manyprickle acathopanax root in traditional Chinese medical science is simplified, the utilization ratio, nutrition and medical value of cordyceps militaris are improved, the yield of cordyceps militaris is improved, the growth cycle of cordyceps militaris is shortened, and the quality of cordyceps militaris is improved; and moreover, implementing conditions are easily achieved.

Description

Technical field [0001] The invention relates to a culture method of Cordyceps militaris. Background technique [0002] Cordyceps militarise (L.) Link is a parasitic hypothermic fungus, also known as Cordyceps militarise. Cordyceps militaris is rich in nutrients and contains cordycepin, adenosine, cordyceps polysaccharide, cordycepic acid, protein, SOD, amino acids, vitamins and a variety of trace elements that are beneficial to the human body; it has the effects of nourishing the lungs and kidneys, hemostasis and resolving phlegm, and is used for a long time. Cough and wheezing, fatigue and coughing up blood, impotence and nocturnal emission, sore waist and knees. Acanthopanax senticosus has the advantages of nourishing qi and nourishing essence, dispelling rheumatism, strengthening muscles and bones, replenishing qi and soothing the nerves. It is used for neurasthenia, hypertension, coronary heart disease, hyperlipidemia, diabetes, arteriosclerosis, etc. It has good curative ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 王满玉
Owner 王满玉
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