Method for constructing gastroparesis rat model

A rat model and gastroparesis technology, which is applied in the field of modeling of animal models for medical research, can solve problems such as hindering research on the mechanism of gastroparesis and lack of modeling methods.

Inactive Publication Date: 2012-01-04
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Problems solved by technology

The above modeling methods have promoted the research of diabetic gastroparesis, but the lack of animal models

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] step:

[0024] (1) choose male SD rats, body weight 210~240g, be divided into experimental group and sham operation group at random, weigh body weight, and inject 10% chloral hydrate anesthetized rat by the dosage of every 100g body weight 0.4ml;

[0025] (2) Fix the animal prone on the stereotaxic instrument, insert the ear rods, the coordinates of the ear rods on both sides are equal to 4 to 6 mm, pull out the tongue with tweezers, hang the upper teeth, clamp the nose clip, and adjust the gear rods -2.3mm ( SD);

[0026] (3) Moisten the hair on the head with alcohol, shave the hair with a razor, disinfect the skin with iodine and fluorine, cut the skin after alcohol deiodination, about 1 to 1.5 cm long, bluntly separate the subcutaneous tissue with the handle of the knife, and expose the skull, 3% H 2 o 2 cauterize until the fontanel is clearly visible;

[0027] (4) Adjust the stereotaxic instrument, align the needle tip of the syringe with the center of the cross ...

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Abstract

The invention relates to a method for constructing a gastroparesis rat model, relating to a method for constructing an animal model for medical research. The method is characterized in that 6-OHDA (Hydroxydopamine) is used for destroying nerve nuclei in a position with coordinates of AP=-5mm, ML=+/-2mm, and DV=-8mm, the dysinsulinism function and the glycometabolism function of an animal are not disturbed, and the method for constructing a non-diabetic gastroparesis rat model which has high modeling rate, low death rate, simpleness in operation, stable results and easiness in standardization is provided, therefore a new tool is provided for systematic research on the nosogenesis of gastroparesis, especially the research on non-diabetic gastroparesis.

Description

Technical field: [0001] The invention relates to a modeling method of an animal model for medical research, in particular to a modeling method of an animal model of gastroparesis. Background technique: [0002] Gastroparesis is a group of clinical syndromes characterized by delayed gastric emptying, mainly manifested as nausea, vomiting, early satiety and a series of symptoms of insufficient gastric motility, without organic lesions of the gastrointestinal tract. Chronic disease is more common, and the symptoms persist or recur for several months or even more than 10 years, which has a certain impact on the quality of life of patients. Accumulating evidence shows that the impact of gastroparesis on mortality and disability cannot be ignored. Gastroparesis can be divided into primary and secondary according to the etiology. Primary gastroparesis, also known as idiopathic gastroparesis, occurs mostly in young women. Secondary gastroparesis is common in patients with diabete...

Claims

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Application Information

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IPC IPC(8): A61D7/00
Inventor 朱进霞冯小燕郑丽飞王建刚
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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