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Method for preparing red ginseng saponins Rg3 group and Rh2 group mixed saponins

A technology for ginsenosides and saponins, applied in the field of Rg3 group mixed saponins, can solve problems such as difficulty, and achieve the effects of low toxicity, simple process method and high activity

Active Publication Date: 2012-02-15
SUNFLOWER PHARM GRP (TIANJIN) DRUG RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the separation and extraction from red ginseng is composed of 20 ( S )-Rg3, 20( R )-Rg3, Rk1 and Rg5 and other four kinds of saponins composed of Rg3 group saponins, separated and extracted by 20 ( S )-Rh2, 20( R )-Rh2, Rk2, Rh3 and other four saponins composed of Rh2 group saponins, very difficult, so far there is no relevant report

Method used

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  • Method for preparing red ginseng saponins Rg3 group and Rh2 group mixed saponins
  • Method for preparing red ginseng saponins Rg3 group and Rh2 group mixed saponins
  • Method for preparing red ginseng saponins Rg3 group and Rh2 group mixed saponins

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Ginseng root contains ginseng's own saponinase (C Zhang, H Yu, Y Bao, L An, F Jin): Chem. Pharm. Bull ., 2001, 49(7), 795-798.; Process BioChem ., 2002, 37, 793-798.). The present invention extracts the extract that does not contain saponin but contains ginseng self-saponinase and other water-soluble substances from ginseng roots (extract of ginseng self-enzyme and water-soluble substances), and Rb1, Rb2, Rc or Rd monomer or its mixture reaction, preparation is by 20( S )-Rg3, 20( R )-Rg3, Rk1, Rg5 and other four kinds of saponins composed of Rg3 group saponins; react with ginsenoside F2, prepared by 20( S )-Rh2, 20( R )-Rh2, Rk2, Rh3 and other four kinds of saponins composed of Rh2 group saponins.

[0030] 1. Preparation of ginseng self-enzyme and water-soluble substance extract of ginseng root:

[0031] Cut 3.5 kg of fresh ginseng (equivalent to 1 kg of dry ginseng) into 2mm thick slices, add 8 liters of 95% ethanol, extract ginsenosides at 50-60°C for 6 h...

Embodiment 2

[0065] Preparation of red ginseng saponins of Rh2 group: the ginsenoside F2 was reacted with the ginseng self-enzymes and extracts of water-soluble substances in Example 1 to prepare mixed saponins of Rh2 group.

[0066] Ginsenoside F2, the raw material required for the preparation of Rh2 group saponins, is described in patents and documents (European Patent EP1354944, US Patent US7,759,101B2, documents H Yu... F Jin: Chem Pharm. Bull ., 2007, 55, 231-235.), that is, the mixture of Rb1, Rb2, Rc and Rd of protopanaxadiol is converted by using ginsenosidase type I enzyme. 100 grams of protopanaxadiol saponins Rb1, Rb2, Rc and Rd mixed saponins are dissolved in 1000 milliliters of 0.02M, pH5 acetic acid buffer, and (obtained by aspergillus fermentation according to the above-mentioned patent and literature method) ginsenoside enzyme Mix 1000 ml of type I enzyme solution, stir and react at 40°C for 20-24 hours, add 2 liters of pre-treated macroporous resin (resin HP-20, product...

Embodiment 3

[0087] Add organic acid in the extract of ginseng self-enzyme and water-soluble substance, can improve conversion protopanaxadiol class Rb1, Rb2, Rc or Rd monomer or its mixture, prepare by 20( S )-Rg3, 20( R )-Rg3 group consisting of Rg3, Rk1 and Rg5 mixed with the effect of rare saponins from red ginseng.

[0088]Add 5 grams of ginsenoside Rb1 to 22 milliliters of water, grind, then add 3 milliliters of the ginseng self-enzyme and water-soluble substance extract of Example 1, add 25 milliliters of water and 10 grams of citric acid, react at 70 ° C for 2 hours, use Diluted with 300 milliliters of water, repeatedly adsorbed saponin on a 100 milliliter macroporous resin column, washed the slag with water, then eluted with 400 milliliters of 70% ethanol, concentrated under reduced pressure, and dried to obtain 2.7 grams of 20( S )-Rg3, 20( R )-Rg3 group mixed saponins composed of Rg3, Rk1 and Rg5.

[0089] Several tests have proved that in the above-mentioned reactions, the r...

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Abstract

The invention discloses a method for preparing red ginseng saponins Rg3 group and Rh2 group mixed saponins. The method comprises the following steps: gen-seng-containing saponins enzyme without containing saponins and extract products of other water-soluble substance are mixed with protopanaxadiol saponins Rb1, Rb2, Rc or Rd monomer or mixed saponins, preparing the Rg3 group saponins composed of 20(S)-Rg3, 20(R)-Rg3, Rk1 and Rg5 red ginseng saponins; reacting with ginsenoside F2, preparing the Rh2 group saponins composed of 20(S)-Rh2, 20(R)-Rh2, Rk2 and Rh3 red ginseng saponins. The red ginseng saponins mixed by Rg3 group and Rh2 group has more safety to human body and higher activity than monomer saponins such as Rg3 and Rh2, and can be used for the exploitation of gen-seng products, health products, cosmetics and other medicines.

Description

technical field [0001] The invention relates to a rare red ginseng saponin 20( S )-Rg3, 20( R )-Rg3, Rk1 and Rg5 four kinds of Rg3 group mixed saponins, composed of red ginseng rare saponins 20 ( S )-Rh2, 20( R The preparation method of the Rh2 mixed saponin composed of four kinds such as )-Rh2, Rk2 and Rh3. Background technique [0002] Ginsenoside 20( S )-Rg3, 20( R )-Rg3, Rk1 and Rg5 etc. four kinds, 20( S )-Rh2, 20( R )-Rh2, Rk2 and Rh3 are the rare saponins of red ginseng present in red ginseng. During the processing of red ginseng, Rb1, Rb2, Rc or Rd of protopanaxadiols in white ginseng are converted into 20( S )-Rg3 and 20( R )-Rg3 saponin, further dehydration 20( S )-Rg3 and 20( R )-The hydroxyl group on the 20-carbon of Rg3 saponin forms the cis-trans structure of Rg5 and Rk1 saponins to form Rg3 group saponins. Similarly, Rb1, Rb2, Rc and Rd of protopanaxadiols in white ginseng during red ginseng processing are transformed into 20( S )-Rh2, 20( R )-Rh...

Claims

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Application Information

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IPC IPC(8): C12P33/20C07J17/00
Inventor 金凤燮鱼红闪
Owner SUNFLOWER PHARM GRP (TIANJIN) DRUG RES INST CO LTD
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