Surface plasma resonance sensing detection system and its detection method

Abstract

The invention is suitable for the technical field of photoelectric detection and provides a surface plasma resonance sensing detection system and its detection method. The surface plasma resonance sensing detection system comprises a light source, a first splitter, a first reflector, a prism, a second reflector, a polarizer, a first imaging device and a controller. With the combination of a Michelson interferometer structure and a scanning mechanism, phase variation information is reflected through a separation distance of the light intensity envelope maximum value of P polarized light, and the variation information of a sample's refractive index is further obtained, so as to avoid tedious phase extraction algorithm and increase practicality. In the meanwhile, the variation information of the refractive index of each point on the sensing surface is recorded by the imagine device to realize multi-point detection with high efficiency. The Michelson interferometer structure is formed by the light source, the first splitter, the first reflector, the second reflector and the first imaging device. The controller is used to control a stepping motor or a servo motor to drive the first reflector to carry out reciprocating motion so as to form the scanning mechanism, therefore endowing the surface plasma resonance sensing detection system with a simple structure and low cost.

Description

technical field [0001] The invention belongs to the technical field of photoelectric detection, and in particular relates to a surface plasmon resonance sensing detection system and a detection method thereof. Background technique [0002] Surface plasmon resonance (Surface Plasmon Resonance, SPR) is an emerging sensing technology, which has the advantages of high sensitivity, high throughput, easy to achieve specific detection and real-time, and does not require labeling, etc., and has been widely used in biological , medicine, food quality and safety, chemical and environmental monitoring and other industries, especially for online real-time detection of biomolecules such as DNA and protein, protein molecules, and drug-protein, nucleic acid-nucleic acid, antigen-antibody, receptor-ligand, etc. interactions among others. [0003] At present, SPR sensing technologies mainly include angle type, spectral type and phase type. Among them, phase type SPR has higher sensitivity a...

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Problems solved by technology

[0004] The purpose of the embodiment of the present invention is to provide a surface plasmon resonance sensing detection system, which aims to solve the problem of difficult phase resolution of the existing phase-type surface plasmon resonance sensing technology

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