Anther-stirring, pollen-collecting and hybrid pollination method of tobacco by using artificial pollination pin
A technology of hybrid pollination and tobacco production, which is applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of incomplete utilization of pollen, large waste of male parent flowers, high production cost of hybrids, etc., and achieve reduction The effect of planting amount, improving pollination effect and improving seed yield
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Embodiment 1
[0022] Embodiment one, taking tobacco hybrid seed propagation as an example, its specific steps are as follows:
[0023] A male parent flower collection, at 8-10 am or 4-6 pm, the pollen tube is elongated, the flower head is partially swollen, the flower tube mouth is slightly pink, the corolla is about to open but not yet open, and the pollen is fully developed However, the flowers that are not scattered and are in the late stage of the elongation of the flower tube of the tobacco plant are gently cut from the flower stalk, and then put into the fresh-keeping equipment for temporary storage, and the storage time does not exceed 24 hours;
[0024] B Prepare pollination utensils, make pollination hooks with semicircular, 2-3cm in diameter, No. 8-10 iron wire, or use other material hooks of the same size. There is a thin rope of about 60-80cm at the end of the pollination hook; the pollination needle is made of bamboo, wood and weed stems, the length is about 10cm, and the diame...
Embodiment 2
[0033] Example 2. In 2009, Eyan No. 1 (the main species of Burley tobacco in Hubei Province) was tested at the China Tobacco Enshi Breeding Base in 2009. Compared with the pollination method of the present invention and the flower-to-flower pollination method, the statistics show that : The seed yield of flower-to-flower pollination method is 5.52kg / mu, the early production cost of tobacco plants is 3088.00 yuan / mu, the cost of pollination period is 2785.00 yuan / mu, the pollination cost of producing 1kg seeds is 504.53 yuan, the early production cost of female parent is 559.42 yuan, The production cost of this product is 236.08 yuan, and the comprehensive cost is 1300.02 yuan; the pollination method seed yield of the present invention is 11.65kg / mu, and the early production cost of tobacco plants is the same as that of the flower-to-flower method, and the pollination period cost is 4224.56 yuan / mu, producing 1kg The pollination cost of seeds is 362.62 yuan, the production cost ...
Embodiment 4
[0035] Embodiment four, in 2010, Burley tobacco MSTN90 was tested at China Tobacco Enshi Breeding Breeding Base, compared with the pollination method of the present invention and the flower-to-flower pollination method, the test data showed: the flower-to-flower pollination method seed yield 4.65kg / mu , the initial production cost of tobacco plants is 3463.37 yuan / mu, the cost of pollination period is 2738.27 yuan / mu, the pollination cost of producing 1kg seeds is 588.88 yuan, the early production cost of female parent is 744.81 yuan, the production cost of male parent is 297.92 yuan, and the comprehensive cost is 1631.61 yuan; Adopt the pollination method of the present invention, seed yield 9.58kg / mu, the early stage production cost of tobacco plant is the same as flower-to-flower method, the cost of pollination period is 4361.31 yuan / mu, the pollination cost of producing 1kg seed is 455.31 yuan, female parent early stage production Cost 361.52 yuan, the production cost of ma...
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