37 results about "Gynophore" patented technology

The invention provides an anther-stirring, pollen-collecting and hybrid pollination method of tobacco by using an artificial pollination pin. The method comprises specific steps that: during 8:00am to 10:00am or 4:00pm to 6:00pm, flowers of tobacco plants in post pollen tube growth periods are cut off and stored; flowers in blooming periods or blossom periods are selected as female parent flowers; during 8:00am to 9:00am or 5:00pm to 6:00pm on a sunny day, anther-stirring, pollen-collecting and hybrid pollination are carried out by using a pollination pin. According to the invention, male parent stamens and female parent pistils are in periods with optimal activities and affinities, such that pollination effect and seed quality are effectively improved; pollens in two pollen sacs of one anther are respectively used for pollinating two female parent flowers, and five anthers of a male parent flower can be used for pollinating 6 to 10 female parent flowers, such that a large amount of male parent pollens are saved, and the plantation amount of male parent tobacco is reduced; the pollination device is easy to manufacture and convenient to use, and the pollination method is simple, such that the seed yield is greatly improved, and the comprehensive cost of seed production is reduced. Therefore, the method is suitable to be applied in tobacco hybridization scientific experiments and tobacco large-area hybrid species propagation and production.

The invention discloses a method for flowering test-tube plantlets of Chinese roses in a test tube and carrying out pistil monosexual flowering and a culture medium thereof. The method comprises the following steps: (1) obtaining rootless test-tube plantlets through subculturing; (2) flowering diploids of the rootless test-tube plantlets of Chinese roses in the test tube; and (3) carrying out induced-flowering on pistils of the diploids of the rootless test-tube plantlets of Chinese roses. According to the method, the flowering rate of diploids of test-tube plantlets can reach about 50%; and diploid flowers and pistil haploid flowers are flowered in a tower mode and exist for 20-35 days, and the flowering rate reaches about 30%.

The invention discloses a cultivating method of red-flesh apples. The cultivating method comprises the following steps in sequence: selecting the branches of apple trees with bud change; grafting the branches with bud change on Fuji apple trees to be used as male parents, wherein the branches with bud change are wooden, and the bast is red; collecting the pollen from the grafted branches; drying at 35 to 37 DEG C for standby; selecting the Fuji apple trees as female parents; removing stamens of the fame parents and remaining pistil at beginning-flower stage; and covering by a bag; pollinating the female parents by the prepared pistil at pistil mature stage; and cultivating the hybrid seeds in current season; and the seeds are flowered and fruited in the fourth years after the seeding, and is one month earlier than Fuji apples in mature stage. According to the cultivating method of the red-flesh apples, natural pistil is collected, and artificial pollination is implemented, so that the fertility rate is increased, natural pollens are adopted, and transgenosis molecules do not exist, so that the red-flesh apples are healthy and have no pollution; and according to the cultivating method, the germination rate and the branching rate are high, the fruit setting rate and the output are high, the yield stabilizing ability is high, and fruits are premature, high-quality and high-output.

The invention discloses an artificial emasculation and hybrid control pollination method of robinia pseudoacacia L.. The method comprises the following steps of pollenator making, selection of hybrid male parent and female parent plants, pollen collecting, artificial pollination, pollinated female plant management and hybrid ripe pod harvesting. According to the artificial emasculation and hybrid control pollination method of the robinia pseudoacacia L., the technical steps of emasculation and pollination for the robinia pseudoacacia L. are described in detail, petals and pistil are not damaged, the artificial pollination ripening rate of the robinia pseudoacacia L. can be significantly increased, and the artificial hybridization breeding success rate of the robinia pseudoacacia L. is increased.

The invention relates to the technical field of quick reproduction of plants, and aims to provide a method for establishing a lily embryogenic callus regeneration system by using pistils as explants. The method comprises the following steps: material acquisition, callus-induced culture medium preparation, explant inoculation and callus induction, small plant regeneration, successive transfer culture of small plants, and transplanting of small plants. The method has the characteristics of low pollution rate, high callus inductivity, large callus conglomeration, favorable callus embryogenic property, high regeneration capacity and high reproducibility; especially, as pistils in the young bud are used as the explants, the pollution rate can be lowered to 0, the callus inductivity of up to 100% can be obtained, the callus conglomeration diameter can be approximate to 1cm after culture for 130 days, the callus conglomeration reproducibility is 100%, the embryogenic property is high, and each pistil can obtain 10-40 small plants on the whole.

The invention discloses a cultivation method for increasing the full-fruit rate of peanuts. The cultivation method comprises the following steps that firstly, main stem growth points are removed; secondly, lateral branches are trimmed; thirdly, leaf fertilizer is supplemented in a centralized mode to increase the gynophore formation rate; fourthly, a chemical regulation agent is sprayed. The cultivation method has the advantages that in the first step, the main stem growth points of peanut seedlings are removed, as a result, the apical dominance can be restrained, and growth of a pair of side branches is promoted; in the second step, main stem growth points of the side branches of the peanut seedlings are removed, triiodobenzoic acid is sprayed, and flower formation and gynophore formation are effectively facilitated; in the third step, monopotassium phosphate is sprayed, as a result, the requirement for phosphorus and potassium during florescence can be met, and flower formation and gynophore formation are effectively promoted; in the fourth step, the chemical regulation agent is sprayed, so that sufficient nutrients for promoting development of legumes are guaranteed; through the combined action of the steps, the full-fruit rate of the peanuts can be increased greatly, three tests prove that the full-fruit rate can be averagely increased by 21.15 percent, and the yield can be averagely increased by 23.08 percent.