A method for establishing a lily embryogenic callus regeneration system with pistil as explant

A technology of embryogenic callus and explants, applied in plant regeneration, botany equipment and methods, horticultural methods, etc., can solve the problems of strict disinfection requirements for tissue culture, affecting the improvement of lily genetic engineering, and destroying rare and endangered species. Achieve the effect of good embryogenicity of callus, high embryogenicity, and tender explants
CN103798138BInactive Publication Date: 2016-08-31ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2016-08-31
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention relates to the technical field of quick reproduction of plants, and aims to provide a method for establishing a lily embryogenic callus regeneration system by using pistils as explants. The method comprises the following steps: material acquisition, callus-induced culture medium preparation, explant inoculation and callus induction, small plant regeneration, successive transfer culture of small plants, and transplanting of small plants. The method has the characteristics of low pollution rate, high callus inductivity, large callus conglomeration, favorable callus embryogenic property, high regeneration capacity and high reproducibility; especially, as pistils in the young bud are used as the explants, the pollution rate can be lowered to 0, the callus inductivity of up to 100% can be obtained, the callus conglomeration diameter can be approximate to 1cm after culture for 130 days, the callus conglomeration reproducibility is 100%, the embryogenic property is high, and each pistil can obtain 10-40 small plants on the whole.
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Description

technical field

[0001] The invention relates to the technical field of rapid plant propagation, in particular to a method for establishing a lily embryogenic callus regeneration system using pistil as an explant. Background technique

[0002] Lilium embryogenic callus regeneration system has the highest proliferation rate and multiplication rate compared with other regeneration systems such as bud regeneration system, which is of great significance for the preservation of excellent germplasm. In addition, the embryogenic callus regeneration system of lily is also the decisive basis of the genetic transformation system of lily, which in turn affects the genetic engineering improvement of lily.

[0003] At present, most of the lily callus regeneration systems are established with the scales of field bulbs as explants. The disadvantage is that the bulbs have been in the soil environment for a long time and carry many germs, so the requirements for tissue culture disinfection ar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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