Method for breeding novel varieties of mellow-type ground-cover chrysanthemum utilizing D.indicum var.aromaticum
A technology of Shennong fragrant chrysanthemum and groundcover chrysanthemum, applied in the field of heterosis utilization, to achieve the effect of extensive management, fewer flowers, and more flowers
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Embodiment 1
[0027] Embodiment 1 Utilizes Shennong fragrant chrysanthemum to cultivate the method for Luzhou-flavor type ground cover chrysanthemum new variety
[0028] 1. Pollen collection and preservation: When the chrysanthemums are budding, use sulfuric acid paper bags to isolate the flower buds of the female local cover chrysanthemum variety 'Pudijin' and the male parent Shennong Xiangju. The pollen of 'Pudijin' and Shennong Xiangju were collected respectively during the full flowering stage, and stored in a dry place at 4°C. Pollen storage is critical for interspecific and intervariety hybrids that do not meet at flowering. In the present invention, the pollen is dried and stored at low temperature (dried on silica gel, stored at 4° C.), which can prolong the lifespan of the pollen to about one month.
[0029] 2. Hybrid pollination: the key to this step is to accurately grasp the fertilization period and the best pollination period of the pistil. For chrysanthemums, 10:00-11:00 in ...
Embodiment 2
[0035] The resistance (drought resistance, saline-alkali tolerance) identification of embodiment 2 hybrids
[0036] The plants with basically the same growth of the female parent 'Pudijin', the male parent Shennong Xiangju, the hybrid 'Huang Qiufang' and the cut chrysanthemum 'Huang Xiufang' were selected for salt tolerance identification. The method of watering different concentrations of salt water was used in the experiment. When processing, first prepare a certain concentration of salt solution (NaCl:Na 2 SO 4 =4: 1, simulate the main salt composition of saline-alkali soil in nature), then water salt water pot by pot according to 0.25%, 0.5%, 0.75% content gradient, the salt water 500ml that every pot pours. All treatments were repeated in pots, each repeated 3 times, arranged in sequence, and clear water was set as the control. After treatment, the salt damage index was investigated every 3 days, and the leaf plasma membrane permeability, malondialdehyde (MDA) content,...
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