Industrialized hybrid seed production for bupleurum
A technology for hybrid seed production and Bupleurum, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of species degradation, mixed germplasm, hinder the industrialization promotion of cultivated Bupleurum, and avoid emasculation. Work, the effect of significant superparent advantage
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Embodiment 1
[0016] This embodiment specifically provides an industrialized Bupleurum hybrid seed production method, comprising the following steps:
[0017] 1) For seedling raising and cultivation, Bupleurum germination, soil preparation and sowing methods refer to the previous patent of the research group (patent name: a high-yield cultivation method of Bupleurum, patent application number: 201910190238.8). The seedlings are thinned at the two-leaf stage after emergence, and the seedling density is 30,000 seedlings per mu.
[0018] Specifically include the following steps:
[0019] Bupleurum germination: From March to April every year (before Qingming Festival), take the dried Bupleurum seeds and soak them in water for 30 minutes, and remove the floating grains and impurities while stirring the seeds in the water. Seeds were placed in 220mg / L gibberellin solution for 24 hours under the environment of 25 degrees. Mix the soaked and dried seeds with the peat soil at a ratio of 1:10 by ma...
Embodiment 2
[0027] Chuanbei Chai No. 1 (CBC 1), Rongxian Bupleurum (BM) and their F1 hybrids (CBC 1 as the female line and BM as the male parent) were used, and they were sown on the farm of Southwest University of Science and Technology in March 2018. The field design was sequential sowing, and 3 biological replicates were set up. The planting area of each genotype is 0.5 mu, the planting density is 60,000 plants / mu, conventional fertilizer and water management and pest control. After the harvest in November of that year, it was found that the production-related indicators were
[0028] The ratio of root dry weight to fresh weight (RDW / RFW) of Chuanbei Bupleurum 1 (CBC 1) group was significantly higher than that of the control group (P<0.01). RL (root length), RT (root diameter), RFW (root fresh weight), RDW (root dry weight) of Rongxian Bupleurum (BM) compared with F1 generation and Chuanbei Bupleurum 1 (CBC 1) generation , RDW / RFW (reduced dry ratio: ratio of root dry weight to fre...
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