Lysinibacillus sp. Gy32 and application thereof

A technology of Bacillus lysinus, GY32, applied in the field of microorganisms

Inactive Publication Date: 2012-02-29
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acquisition of microorganisms with the function of degrading polybrominated diphenyl ethers undoubtedly brings hope to the solution of these pollution problems

Method used

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  • Lysinibacillus sp. Gy32 and application thereof
  • Lysinibacillus sp. Gy32 and application thereof
  • Lysinibacillus sp. Gy32 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 20g of riverbed sediment from an electronic waste centralized treatment area in Shantou, Guangdong, add 80mL of sterile water to stir and disperse, and then sieve to remove plant residues and large particles; the filtrate is then centrifuged at 2000×g at room temperature for 2 minutes to remove small particles ; The obtained supernatant was centrifuged at 6000×g at room temperature for 10 minutes to collect the precipitate; the precipitate was added to 80 mL of sterile water, washed and centrifuged three times. The finally obtained precipitate was used as a seed source and inoculated into inorganic salt medium (Na 2 HPO 4 5.7mM, KH 2 PO 4 3.3mM, NH 4 Cl 18.0mM, yeast extract 1g / L, sodium succinate 20mM, sodium formate 20mM, sodium lactate 20mM, trace elements and vitamins, trace elements: 1.01mmol / L MgSO 4 , 0.5g / L MnSO 4 , 0.1g / L ZnSO 4 , 0.01g / LCuSO 4 , 0.01g / L AlK(SO 4 ) 2 , 1.0g / L NaCl, 0.1g / L FeCl 2 , 0.1g / L CaCl 2 and CoCl 2 0.1g / L. Vitamins: 2mg...

Embodiment 2

[0031] Example 2: Debromination degradation experiment of 5 μmol / LBDE209 by Lysinibacillus sp. GY32 under facultative anaerobic conditions.

[0032] Culture system composition: 5.7mmol / L Na 2 HPO 4 .12H 2 O, 3.3mmol / LKH 2 PO 4 , 18.0mmol / LNH 4 Cl, 20mmol / L sodium formate, 20mmol / L sodium lactate, 40mmol / L sodium succinate, 0.1% yeast juice, 5μmol / L BDE209, the balance is water, pH7.0. The Lysinibacillus sp. GY32 bacterial solution was inserted into the above-mentioned culture system according to the inoculum amount of 5%, and after 21 days of culture on a shaking table in the dark, the debromination amount of BDE209 was measured with an ion chromatograph DIONEX ICS-1500. The average concentration of bromide ion detected in the culture system was 351.9 μg / L, which indicated that Lysinibacillus sp. GY32 had the ability to degrade BDE209.

Embodiment 3

[0033] Example 3: Debromination degradation experiment of 5 μmol / LBDE209 by Lysinibacillus sp. GY32 under anaerobic conditions.

[0034] Culture system composition: 5.7mmol / L Na 2 HPO 4 12H 2 O, 3.3mmol / L KH 2 PO 4 , 18.0mmol / L NH 4 Cl, 20mmol / L sodium formate, 20mmol / L sodium lactate, 40mmol / L sodium succinate, 0.1% yeast juice, 5μmol / L BDE209, the balance is water, pH7.0. Introduce the Lysinibacillus sp. GY32 bacterial solution into the above-mentioned culture system according to the inoculum amount of 5%, seal it with an aluminum cover, keep it in the dark for 21 days, and use an ion chromatograph DIONEX ICS-1500 to measure the debromination of BDE209 quantity. The bromide ion concentration detected in the culture system reached an average of 28.7 μg / L, which indicated that Lysinibacillus sp. GY32 had the ability to degrade BDE209.

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Abstract

The invention discloses a Lysinibacillus sp. GY32 and the application thereof. The Lysinibacillus sp. GY32 is collected in the CCTCC (China Center for Type Culture Collection) on September 7, 2011, address: Wuhan University, Wuhan, China, and the collection number is CCTCC NO: M 2011307. The invention provides a new breed: Lysinibacillus sp. GY32, capable of degrading PolyBrominated Diphenyl Ethers BDE209, so that the degrading bacteria for managing the PolyBrominated Diphenyl Ethers BDE209 are provided.

Description

Technical field: [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Lysinibacillus sp. GY32 and its application in degrading decabromodiphenyl ether. Background technique: [0002] Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants that are widely used in electrical and electronic products due to their excellent flame retardancy. In recent years, with the rapid development of the electronic and electrical industry, a large amount of electronic waste has been dismantled disorderly, and PBDEs with ecophysiological toxicity and bioaccumulation toxicity are released into the water and soil environment, which has caused serious impacts on the ecological environment and the health of organisms. and hazards. Faced with the ecological and environmental problems caused by polybrominated diphenyl ether pollution, people are actively exploring various solutions. Appropriate microbial treatment can achieve comple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/28
Inventor 陈杏娟朱春节许玫英孙国萍郭俊
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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