Method for evaluating influence of environmental single factor on transgene plant gene flow

Abstract

The invention provides a method for evaluating the influence of an environmental single factor on transgene plant gene flow. According to the method, factors which may influence the gene flow are extracted from natural environment by manual simulation at room temperature. The natural environment is separated and the gene flow test is carried out under a single factor so as to determine the influence of the single factor on the gene flow. With the combination of real measured values under different regional and environmental factors, main factors which influence the gene flow in the locality can be better determined and an effective predetermined plan for the planting of transgene plants is made, so as to minimize test repetitions to some extent and save test cost. In addition, different from wagon-wheel bulk sampling point F1 sampling analysis, the method provided by the invention requires less occupation space, and can be used to reduce F1 sampling amount and greatly decrease the workload of test post-detection and analysis.

Description

Technical field [0001] The invention relates to a method for evaluating the ecological safety of transgenic plants, in particular to a method for evaluating the impact of environmental single factors on gene drift of transgenic plants. Background technique [0002] While large-scale commercialization of genetically modified plants, their ecological safety and other issues have also caused widespread controversy and concern worldwide. In theory, there are roughly two ways of gene drift in transgenic plants: one is gene drift in the usual sense, which is mainly to transfer genes between groups of the same species or between relative species that can be mated through sexual crossing. According to different media, the phenomenon includes pollen-mediated, seed-mediated and asexual reproduction organ-mediated gene drift; the second is gene-level transfer, which refers to the transfer of genes in individuals of different species through non-sexual reproduction. The phenomenon of inter-...

AI Technical Summary

Problems solved by technology

Although the wagon-wheel method can infer the influence of environmental factors on pollen drift to a certain extent, the content is vague, and the data is the result of mixed environmental factors, and the trend description is not clear

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