Method for efficiently preparing natural abscisic acid

A natural abscisic acid, high-efficiency technology, applied in the field of microbial fermentation, can solve the problems of low production cost, high production cost, low fermentation yield, etc., achieve the effect of reducing energy consumption, reducing production cost, and improving dissolved oxygen status

Active Publication Date: 2012-04-04
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the deficiencies in the production of natural abscisic acid known in the prior art, such as low fermentation yield, high energy consumption and high production c

Method used

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  • Method for efficiently preparing natural abscisic acid
  • Method for efficiently preparing natural abscisic acid
  • Method for efficiently preparing natural abscisic acid

Examples

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Effect test

Embodiment 1

[0116] Use 25 1000ml Erlenmeyer flasks, each with 300ml medium A, sterilize at 120°C, inoculate the activated abscisic acid high-yielding strain Botrytis cinerea TBC-20 (CGMCC No.4645) spore suspension after cooling, and place at 25°C Shake flasks were incubated at temperature for 24-46 hours. Inoculate the cultured primary seed liquid into a 100 liter fermenter (secondary seed tank) with 50 liters of medium B inside by 13%-15% inoculum, and cultivate it with aeration and stirring at a temperature of 26°C-28°C for 24 -40 hours.

[0117]Use a 1-ton fermenter for three-stage fermentation. The medium C in the tank is about 500L. After sterilizing with conventional high-pressure hot steam sterilization, inoculate the seed liquid of the second-stage tank at 10%-15% of the inoculum. 26°C-27 After 20-40 hours of fermentation with aeration and stirring at ℃, Tween-20 was added at one time, and the amount added was 0.05% of the volume of the fermentation broth, and then feeding medium...

Embodiment 2

[0123] Use 50 1000ml Erlenmeyer flasks, each with 300ml medium A, sterilize at 120°C, inoculate the activated abscisic acid high-yielding strain Botrytis cinerea TBC-20 (CGMCC No.4645) spore suspension after cooling, and place at 23°C Shake flasks were incubated at -25°C for 24-33 hours. Inoculate the cultured primary seed liquid into a 200 liter fermenter (secondary seed tank) with 100 liters of medium B inside by 15%-18% inoculation amount, and cultivate it with aeration and stirring at a temperature of 24°C-26°C for 24 -30 hours.

[0124] Use a 1-ton fermenter for the third-stage fermentation. The medium C in the tank is about 500L. After sterilization by conventional high-pressure hot steam sterilization, inoculate the seed liquid of the second-stage tank according to the inoculation amount of 20%-25%. After 15-24 hours of fermentation with aeration and stirring at 26°C, Tween-20 was added at one time, and the amount added was 0.08% of the volume of the fermentation broth...

Embodiment 3

[0130] Use 30 1000ml Erlenmeyer flasks, each containing 300ml medium A, sterilize at 120°C, inoculate the activated abscisic acid high-yielding strain Botrytis cinerea TBC-10 (CGMCC No.1889) spore suspension after cooling, and place at 27°C Next, shake flasks for 24-33 hours. Inoculate the cultured primary seed liquid into a 100 liter fermenter (secondary seed tank) with 50 liters of medium B inside by 15%-20% inoculation amount, and cultivate it with aeration and stirring at a temperature of 24°C-27°C for 24 -30 hours.

[0131] Use a 1-ton fermenter for the third-stage tank fermentation, and the medium C in the tank is about 500L. After sterilizing with conventional high-pressure steam sterilization, inoculate the seed liquid of the second-stage tank according to the inoculation amount of 10%-15%. After fermenting for 15-24 hours with aeration and stirring at 28°C, add Tween-20 at one time, the addition amount is 0.1% of the volume of the fermentation broth, and at the same ...

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Abstract

The invention belongs to the technical field of microbial fermentation and relates to a method for efficiently preparing natural abscisic acid through adding acetylcoenzyme A as precursor substances for strains to synthesize the abscisic acid in a flowing way in the fermentation process and simultaneously adding biosurfactant tween-20 for improving the dissolved oxygen condition of the fermentation liquid. The method comprises the following steps that fungi capable of producing the natural abscisic acid are cultured in a first stage liquid culture medium; the cultured first stage seed liquid is inoculated onto a second stage liquid culture medium for culture, supplementing materials are added to a third stage liquid culture medium in the flowing way, simultaneously, the tween-20 is added,and the acetylcoenzyme A is added in the flowing way for fermentation culture; and the obtained abscisic acid is collected from the fermentation culture liquid. The method has the advantages that theoxygen utilization rate of the strains and the acid yield can be improved, so the strains can be used for producing the abscisic acid at higher substrate conversion rate and higher product synthesis velocity, the yield and the production efficiency are improved, the energy consumption is reduced, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and relates to a new method for fermenting and producing natural abscisic acid (ABA). Background technique [0002] Abscisic acid (ABA) is one of the five major plant hormones found in the world. Natural abscisic acid has a strong regulatory activity on the growth and development of crops, can promote the maturity and development of fruits, cereals, and beans, can greatly improve their yield and quality, and can greatly enhance their cold resistance, drought resistance and resistance. Salt-alkali ability, so it has broad application prospects. At present, the research on abscisic acid in the basic field has reached the level of plant cells and genetic engineering. However, since the optical configuration of natural abscisic acid present in plants is only (S)-(+)-abscisic acid, the production cost of pure (S)-(+)-abscisic acid is extremely high and the price is expensive. The syn...

Claims

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Application Information

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IPC IPC(8): C12P7/42C12R1/645
Inventor 谭红周金燕钟娟杨杰肖亮
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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