Method for building sequencing library by hybridization

A library and genome library technology, applied in the field of second-generation sequencing technology, labeling technology, realizing sequence capture of multiple samples in the same reaction system, solexa sequencing technology, hybridization-based sequence capture technology, can solve the waste of sequencing data , poor sequence capture effect, non-target sequences, etc., to avoid data waste, avoid the reduction of sequencing length, and improve the efficiency of introduction
CN102409047AActive Publication Date: 2012-04-11BGI TECH SOLUTIONS
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
BGI TECH SOLUTIONS
Publication Date
2012-04-11

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Abstract

The invention provides indexes for DNA sequencing, especially for a sequence acquisition technology, and a method for introducing the indexes by adopting a PCR (polymerase chain reaction) method. The invention also provides adapter block sequences for blocking adapters and application of the adapter block sequences in a NimbleGen chip hybridization system, an Agilent liquid hybridization system and a NimbleGen EZ liquid hybridization system. The invention further provides a method for building a library and / or sequencing by using the indexes and / or adapter block sequence, and the library built by the method.
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Description

technical field

[0001] The present invention relates to the technical field of nucleic acid sequencing, in particular to the field of sequence capture technology, especially the sequence capture technology based on hybridization. In addition, the present invention also relates to labeling technology and a method for realizing sequence capture of multiple samples in the same reaction system. The method of the present invention is particularly suitable for the second generation sequencing technology, especially the solexa sequencing technology. The methods of the invention are useful in genomics research. Background technique

[0002] The second-generation sequencing technology represented by Illumina solexa, AB Solid and Roche 454 has greatly reduced the cost of sequencing, has developed rapidly in recent years, and has become an important tool for genomics research. Compared with the sanger sequencing technology of the chain termination method, the second generation sequen...

Claims

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