33results about How to "Improve introduction efficiency" patented technology

The invention relates to a preparation method of a magnetic cadmium ion surface imprinted polymer, which comprises the following steps: synthesizing magnetic Fe3O4@SiO2 microspheres through a coprecipitation method and a sol-gel method, and treating with acid to increase the surface hydroxyl content; introducing polymerizable double bonds onto the surfaces of the Fe3O4@SiO2 microspheres through a two-step method, namely grafting amino groups onto the surfaces of the microspheres by using aminosilane coupling agent, and generating the double bonds through reaction between acid anhydride and the amino groups; and finally, performing polymerization reaction by taking the Fe3O4@SiO2 microspheres as carriers, taking Cd(II) as a template, taking ethylene glycol dimethacrylate as crosslinking agent and taking methacrylic acid and salicylaldoxime as monomers, and washing off the template Cd(II) after the reaction, thus obtaining a polymer layer with a large number of Cd(II) imprinted holes distributed on the surfaces of the Fe3O4@SiO2 microspheres. The magnetic cadmium ion surface imprinted polymer provided by the invention can be used for separation and removal of cadmium ions.

The present invention discloses a method for manufacturing rare earth (RE) doped optical fibers by using silica nanoparticles coated with rare earth oxides as a precursor material. More specifically, the method of the present invention includes the following steps: A stable dispersion (sol) of silica nanoparticles coated with a rare earth oxide is prepared at ambient temperature; the inner surface of a silica glass tube is coated with the silica nanoparticles using a dip coating technique or any other conventional method. A thin coating of silica sol containing appropriate dopants selected from Ge, Al, P, etc.; then using MCVD technology to make a preform of optical fiber, and then forming an optical fiber of desired shape. The novelty of the present invention is that when forming the fiber core, the step of forming a loose powder layer at high temperature in the fused silica glass tube in the CVD process is omitted, and the introduction of rare earth ions into the loose powder layer by solution doping technology or other methods is omitted. The step in the powder layer, the direct addition of rare earth oxides in the sol avoids the formation of rare earth ion crystallites and clusters, and prevents compositional changes including changes in the concentration of rare earths in the core, thereby greatly increasing the process reproducibility and reliability, in addition, at ambient temperature the Ge(OET) 4 The addition of silica sol above reduces the GeCl required to achieve the desired numerical aperture at high temperature 4 amount.

The invention discloses a small portable heavy metal detection device. The small portable heavy metal detection device comprises an enrichment evaporator, a low-temperature plasma exciter and a spectrum detection device, wherein the enrichment evaporator comprises a reaction cavity; the upper part and the lower part of the reaction cavity are provided with a sample inlet and a waste liquid outlet, respectively; the reaction cavity is internally provided with a reaction wire and a reference wire; the reference wire is only connected with the positive electrode of a power supply; two ends of the reaction wire are connected with the positive electrode and the negative electrode of the power supply, respectively; the low-temperature plasma exciter comprises an excitation cavity; the top end of the excitation cavity is provided with an observation window and a waste gas outlet; two ends of the excitation cavity are connected with a power supply of a neon lamp; the lower part of the excitation cavity is communicated with the upper part of the reaction cavity; the spectrum detection device comprises a CCD (charge coupled device) spectrograph and an optical fiber probe which are connected with each other; the optical fiber probe is installed on the observation window of the excitation cavity; the CCD spectrograph can be connected with a computer. The small portable heavy metal detection device is simple in structure, low in cost, small in size and convenient to carry, can enrich heavy metal in a to-be-detected liquid effectively, can excite detection and can achieve field analysis of detection data.

Disclosed are a group of isolated nucleic acid tags for constructing a genome sequencing library, oligonucleotides, blocking sequences, a kit, a method for constructing a genome sequencing library, the genome sequencing library constructed, a method for sequencing a particular region in the genome of a sample, and a method for sequencing the genomes of a plurality of samples. The group of isolated nucleic acid tags is comprised of nucleotides as set forth in SEQ ID NO: (165+M), where M = any integer between 1-159.

The present invention relates to a composition for intracellular delivery containing an adenovirus protein VI-derived peptide and an anti-cancer pharmaceutical composition containing the same. According to the present invention, the use of the peptide or peptide-polymer composite of the present invention improves intracellular delivery efficiency of a nucleic acid, a peptide, a poly-peptide, an antibody, a chemical material, or a virus. Therefore, the peptide or peptide-polymer composite of the present invention can be favorably used as an intracellular delivery system for various therapeutics.

In order to provide a novel method for safely and efficiently introducing nucleic acids, proteins or other target substances into T cells or other immune cells, the present invention provides a system for delivering target substances to immune cells comprising a combination of an ultrasound generating device and an aqueous solution or water of ultra-fine bubbles, the aqueous ultrafine bubble solution or ultrafine bubble water includes ultrafine bubbles having an average diameter of 200 nm or less and not containing phospholipids. The present invention provides, inter alia, a method for improving the delivery ability of nucleic acids or proteins to immune cells using ultrasound and the above-mentioned ultrafine bubble water, etc.

The invention discloses a preparation method of a magnetic lead ion surface imprinting polymer. The method comprises the following steps: firstly, synthesizing magnetic Fe3O4@SiO2 microspheres by using a 'coprecipitation method' and a 'sol-gel method', processing by using an acid, so as to improve the hydroxy content of the surface; then leading polymerizable double bonds to the surfaces of the Fe3O4@SiO2 microspheres by using a 'two-step process', firstly, grafting amino on the surfaces of the microspheres by using an amino silane coupling agent; generating double bonds by using reaction of anhydride and amino; finally taking the Fe3O4@SiO2 microspheres as a carrier, the Pb(II) as a template, ethylene glycol dimethacrylate as a crosslinking agent and methacrylic acid and salicylaldoxime as monomers to carry out polymerization reaction; and washing off the template Pb(II) after reaction is ended, so as to obtain a polymer layer with a lot of Pb(II) imprinting cavities distributed at the surfaces of the Fe3O4@SiO2 microspheres. The magnetic lead ion surface imprinting polymer disclosed by the invention can be applied to separation and removal of lead ions.

The invention discloses a radio-frequency glow discharge ionization device comprising a closed ionization chamber, a mass spectrometer and a device main body located in the ionization chamber. The device main body comprises a copper block fixing seat which contains a copper block and a sample fixing seat which contains a sample, the copper block is connected to an external circuit through a direct current automatic biasing voltage interface on the ionization chamber, and the sample is connected to a radio-frequency power source through a radio-frequency signal importing interface. The copper block fixing seat and the sample fixing seat are arranged in an opposite mode between the copper block and the sample to form an ionization area. A repulsion board which puts plasma in the ionization area into the mass spectrometer is arranged on one side of the ionization area, and the repulsion board is connected to a high direct voltage power source through a repulsion voltage importing interface on the ionization chamber. The radio-frequency glow discharge ionization device cannot be limited by the material of conductor or semiconductor or nonconductor, has the advantages of high in ionization efficiency and ionization importing efficiency, and is characterized by wide detection applying range, high sensitivity and low detection limit.