32results about How to "Improve introduction efficiency" patented technology

The invention relates to a preparation method of a magnetic cadmium ion surface imprinted polymer, which comprises the following steps: synthesizing magnetic Fe3O4@SiO2 microspheres through a coprecipitation method and a sol-gel method, and treating with acid to increase the surface hydroxyl content; introducing polymerizable double bonds onto the surfaces of the Fe3O4@SiO2 microspheres through a two-step method, namely grafting amino groups onto the surfaces of the microspheres by using aminosilane coupling agent, and generating the double bonds through reaction between acid anhydride and the amino groups; and finally, performing polymerization reaction by taking the Fe3O4@SiO2 microspheres as carriers, taking Cd(II) as a template, taking ethylene glycol dimethacrylate as crosslinking agent and taking methacrylic acid and salicylaldoxime as monomers, and washing off the template Cd(II) after the reaction, thus obtaining a polymer layer with a large number of Cd(II) imprinted holes distributed on the surfaces of the Fe3O4@SiO2 microspheres. The magnetic cadmium ion surface imprinted polymer provided by the invention can be used for separation and removal of cadmium ions.

The invention relates to a preparation method of a magnetic zinc ion surface-imprinted polymer, which comprises the following steps: synthesizing magnetic Fe3O4@SiO2 microspheres by a coprecipitation process and a sol-gel process, and treating with acid to enhance the surface hydroxy content; introducing a polymerizable double bond onto the surface of the Fe3O4@SiO2 microspheres by a two-step process, i.e. grafting amino group onto the microsphere surface by using an aminosilane coupling agent, and reacting acid anhydride with the amino group to generate the double bond; and finally, carrying out polymerization reaction by using the Fe3O4@SiO2 microspheres as a carrier, Zn (II) as a template, ethylene glycol dimethacrylate as a crosslinking agent and methacrylic acid and salicylaldoxime as monomers, and after the reaction finishes, washing out the template Zn (II) to obtain the polymer layer in which abundant Zn (II) imprinted holes are distributed on the Fe3O4@SiO2 microsphere surface. The magnetic zinc ion surface-imprinted polymer can be used for separating and removing zinc ions.

The present invention discloses a process for making rare earth (RE) doped optical fibre by using RE oxide coated silica nanoparticles as the precursor materia, more particularly the method of the present invention involves preparation of stable dispersions (sol) of RE oxide coated silica nanoparticles at ambient temperature and applying a thin coating on the inner surface of silica glass tube following dip coating technique or any other conventional methods, of the said silica sol containing suitable dopants selected from Ge, Al, P, etc., the coated tubes were further processed into optical preforms by following MCVD technique and fiberised in desired configuration, the novelty lies in eliminating the step of the formation of porous soot layer at high temperature by CVD process inside a fused silica glass tube for formation of the core and also in the elimination of the incorporation of the rare earth ions into the porous soot layer following the solution doping technique or other conventional methods, the direct addition of RE oxides in the sol eliminates the formation of microcrystalites and clusters of rare earth ions and prevents change in composition including variation of RE concentration in the core which results in increase in the reproducibility and reliability of the process to a great extent, further the addition of Ge(OET)4 at ambient temperature in the silica sol reduces the quantity of GeCl4 which is required at high temperature to achieve the desired Numerical Aperture.

The invention provides indexes for DNA sequencing, especially for a sequence acquisition technology, and a method for introducing the indexes by adopting a PCR (polymerase chain reaction) method. The invention also provides adapter block sequences for blocking adapters and application of the adapter block sequences in a NimbleGen chip hybridization system, an Agilent liquid hybridization system and a NimbleGen EZ liquid hybridization system. The invention further provides a method for building a library and/or sequencing by using the indexes and/or adapter block sequence, and the library built by the method.

The invention discloses a preparation method of a modified polyether-ether-ketone resin-based sizing agent for carbon fiber, and belongs to the technical field of carbon fiber sizing agents. The method comprises the steps that an appropriate amount of polyether-ether-ketone powder is taken for first chemical modification, and a polyether-ether-ketone derivative is obtained; a carbon nano materialis subjected to second chemical modification so as to obtain a carbon nano modified product; the polyether-ethe- ketone derivative is dissolved into an organic solvent, the carbon nano modified product is added, and the sizing agent is obtained after uniform dispersion. Accordingly, the high temperature resistance of the carbon fiber/polyether-ether-ketone (CF/PEEK) composite material sizing agentis improved, the compatibility of the sizing agent and the polyether-ether-ketone (PEEK) resin is good, interface bonding of the carbon fiber and a resin matrix can be significantly improved, and thetechnical effect of the mechanical property of the composite material is improved.

The invention discloses a preparation method of a carbon fiber enhanced carbon-silicon carbide friction material, belonging to the preparation field of carbon fiber enhanced carbon-silicon carbide composite materials. The preparation method comprises the following steps: (1) weaving a carbon fiber preform for formation; (2) carrying out high-temperature thermal treatment on the carbon fiber preform; (3) preparing an asphalt-resin mixed impregnant; (4) carrying out dipping, curing and carbonization on the asphalt-resin mixed impregnant; (5) preparing resin/carbon powder dipping precursor; (6) carrying out dipping, curing and carbonization on the resin/carbon powder dipping precursor; (7) carrying out high-temperature thermal treatment on a porous carbon/carbon blank; and (8) carrying out fusion siliconing on the porous carbon/carbon blank, so as to obtain the carbon fiber enhanced carbon-silicon carbide friction material. The preparation method is simple and controllable in process, short in preparation period and strong material structure designability, and the prepared friction material can be applied to automobile brake discs (pieces), high-speed train brake shoes and magnetically levitated train runners.

The invention relates to a preparation method of a Mo-SBA-15 molecular sieve. The compounding steps include adding a mesoporous material SBA-15, which is self-assembled in a strong-acid system but not subjected to hydro-thermal treatment, into a solution containing a VIB-family metal precursor, adjusting the reaction system pH value to 0-2, and subjecting the solution to high-temperature hydrothermal crystallization to obtain a sample, namely the SBA-15 molecular sieve containing VIB metal. The obtained Mo-SBA-15 molecular sieve has the advantages of high loading capacity, high dispersity and highly-ordered mesopores. By taking the Mo-SBA-15 molecular sieve as a carrier, a catalyst with high large specific surface area, excellent reactive metal dispersity, high acidity and excellent hydrodesulphurization performance can be obtained only via a VIII-family metal impregnation process.

The invention relates to sugar-sensitive intelligent hydrogel based on a gold surface and a synthesis method thereof. The synthesis method of the gold surface-based sugar-sensitive intelligent hydrogel comprises the following three steps of pre-treating a gold sheet so that the surface of the gold sheet is provided with -OH, efficiently introducing double bonds on the surface of the gold sheet orderly by a silane coupling agent and an acid anhydride, synthesizing the hydrogel on the surface of the gold sheet from acryamidophenylboronic acid as a monomer, a glucose molecule as a template and N, N'-methylene bisacrylamide as a cross-linking agent by an ultraviolet light-initiated polymerization reaction, and after the reaction, removing the glucose molecule by washing so that a large amount of glucose molecular imprinting cavities are formed on a hydrogel membrane on the surface of the gold sheet. The sugar-sensitive intelligent hydrogel based on a gold surface will be used in the field of biological signal detection and realizes dynamic identification and detection of glucose and other physiological indexes under the human physiological conditions.

The invention relates to the compound used in element formation the volatile matter and this compound molecule composition is HnBmMp and H represents hydrogen n is not less than six and not greater than thirty and B represents boron m is not less than 2 and not greater than 20 and M presents lithium, natrium, kalium, ammonium, chorine, bromine, iodine or organic molecule or organic ion p is not less than 0 and not greater than 3. Adopt one kind or multiple kinds mixture of the compound liquor to react with the water liquor with the measuring element is no only make the arsenic, antimony, bismuth, germanium, tin, lead, selenium, tellurium, hydrargyrum which can easy to form the hydride in tradition formed the volatile matter also makes the other multiple tens elements (gold, silver, colalt, palladium, manganese, calcium and so on) formed the hydride. The compound can apply in the atomic spectrum analysis of the sample inlet so it can enhance the sensitivity of analysis detection and reduces the detection limit also can used in chemistry vapour deposition and the production of inorganic metallic-membrane plating gassy precursor.

The invention discloses a pyrrole lysyl-tRNA synthetase mutant and application thereof. The mutant is obtained by mutating an amino acid sequence shown in SEQ ID NO.3 at a 31st site, a 56th site, a 100th site, a 311th site and a 313th site. According to the invention, the MbPylRS sequence is modified, so that the tRNA recognition rate is improved, and the introduction efficiency of unnatural amino acid is further improved. According to verification, the combination efficiency of mutation of different non-natural amino acids MbPylRS is higher than that of MmPylRS, the introduction efficiency of the modified MbPylRS to ten non-natural amino acids is improved by 3-6 times compared with that of MbPylRS (N311A/C313A), and the non-natural amino acids are successfully used for expression of GFP protein. The method solves the problem of low introduction efficiency of non-natural amino acid, and is convenient to popularize and apply.

The invention discloses a guide-in conveying tool and method of a nuclear power station spent fuel framework. The tool comprises a conveying rail and a conveying trolley operating on the conveying rail. The conveying rail is laid on a conveying passage on the outer side of a fuel building and penetrates a fuel building door to extend to a turning table in the fuel building. The conveying trolley comprises tank wheels matched with the rails and a movable conveying platform supported on the tank wheels. The method is a method for using the tool to convey the spent fuel framework into the fuel building. Compared with the prior art, the guide-in conveying tool and method has the advantages that a trailer needs not to be occupied for a long time, and the tool and method is high in cleanness and work efficiency, and the like.