Culture media for peony high frequency embryogenic callus differentiation, and culture method thereof

A technology of embryogenic callus and differentiation medium, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low differentiation rate of embryogenic buds of peony callus, and achieve compact texture and convenient material extraction , the effect of not easy browning

Inactive Publication Date: 2012-05-09
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem of low embryogenic bud differentiation rate of callus of Paeoniae alba remains to be solved

Method used

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  • Culture media for peony high frequency embryogenic callus differentiation, and culture method thereof
  • Culture media for peony high frequency embryogenic callus differentiation, and culture method thereof
  • Culture media for peony high frequency embryogenic callus differentiation, and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Collect the naturally matured seeds of 'Zifengyu' and store them in the refrigerator for later use.

[0029] (1) Material collection: The seeds to be tested were sterilized with 1% NaClO for 10 min, rinsed with tap water, soaked in water for 1 day, then sterilized with 75% alcohol for 30 s, sterilized with 0.1% mercuric chloride for 8 min, rinsed with sterile water After cleaning, put it on an ultra-clean workbench, peel off the seed coat and endosperm in turn, and pick out the seed embryo with a dissecting needle.

[0030] (2) Culture medium formula:

[0031] The improved 1 / 2 MS formula is to improve the content of macroelements in MS medium to KNO 3 62.5 mg / L, NH 4 NO 3 125 mg / L, MgSO 4 ·7H 2 O 62.5 mg / L, KH 2 PO 4 137.5 mg / L, CaCl 2 2H 2 O 125 mg / L, and the contents of other trace elements, iron salts and organic components remained unchanged.

[0032] ① Inoculate the seed embryos in the following three media for inducing embryogenic callus:

[0033] 1)...

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Abstract

The present invention relates to a culture media for peony high frequency embryogenic callus differentiation, and a culture method thereof. The culture method comprises steps of material selecting, seed embryo sterilizing and separating, culture media preparing, callus differentiation and embryo bud differentiation. The culture media for the peony callus differentiation is prepared by adding 0.5-2.0 mg/L of NAA, 0.1-0.5 mg/L of TDZ, 30 g/L of sucrose and 6 g/L of agar to a modified 1/2MS basic culture media, wherein the pH value is 5.8. The culture media for the peony embryo bud differentiation is prepared by adding 1.0-5.0 mg/L of TDZ, 100 g/L of sucrose and 6 g/L of agar to the modified 1/2MS basic culture media, wherein the pH value is 5.8. The culture media and the culture method of the present invention have the following advantages that: the raw materials are easily obtained; the callus rate is high; the browning rate is low; the embryo bud differentiation rate is high; the generesearch and the gene application requirements can be met.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a culture medium and a culture method for high-frequency embryogenic callus differentiation of peony. Background technique [0002] Peony is a traditional famous flower in my country. Compared with other flowers, the tissue culture of peony is more difficult. Previous studies have shown that using leaves, petioles, stems, etc. as explants can induce callus and differentiate into buds, but the test materials are heavily polluted, callus is often browned, and the rate of bud differentiation is low. It is far from the requirements of the peony genetic transformation regeneration system. [0003] A culture medium is disclosed in the Chinese patent application "Paeoniae in Vitro Tissue Culture Method" (publication number CN1675992): 1 / 2MS medium + V C 50mg / L, pH 5.6. The success of this application lies in the establishment of a method for the asexual propagation of shoot ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陶俊赵大球薛银芳韩晨霞葛金涛
Owner YANGZHOU UNIV
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