Method of capturing cell fluid and analyzing components thereof while observing cell and system for capturing and analyzing cell fluid

Abstract

It is intended to provide a means of quickly clarifying the dynamics and molecular mechanism of a biological phenomenon by, at least in a single cell, enabling the convenient and quick detection of molecules in a fluid contained in the cell and simultaneously observing the cell dynamics. At the same time, it is aimed at providing a means of searching for an active molecule providing a certain state of a cell from among a number of the detected molecules. The fluid contained in a single cell is directly sucked through a capillary and then loaded directly on an analyzer. This procedure is carried out under a magnifying glass such as a microscope while observing the cell. In the case of employing mass spectrometry, in particular, the fluid contained in the cell is captured by directly sucking through an ionization capillary for a nano-spray, mixed with an ionization-assisting solvent, and then directly introduced into a mass spectrometer by the direct spraying method. Thus, the molecular components of the inner fluid are detected even from a single cell. By analyzing the thus captured molecular peaks among cell data groups in different states with the use of differential analysis or t-calibration, a peak, i.e., a molecule that is specific to a certain state is identified.

Description

technical field

[0001] The present invention relates to a real-time collection of intracellular solution and component analysis method and collection of cell fluid and analysis device accompanying cell observation. Background technique

[0002] The molecular mechanism in cells is the fundamental substance that causes life phenomena, and it is an eternal subject that life sciences should clarify. But so far, there is no analytical method that can detect and identify the huge molecular groups and ion groups of the cells in real time or over time while directly observing the dynamic changes of living cells, and analyze key molecules. , and then quickly clarify the relationship between the molecular structure and the observation of phenomena such as cell morphology. If such a method and device can be developed, the time to discover not only life phenomena but also disease markers and marker molecules for disease detection, or the time to discover candidate molecules that can be...

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