Compound fingerprint atlas model used in early-stage gastric cancer diagnosis/early warning, and establishing method thereof

一种指纹图谱、早期胃癌的技术，应用在生物系统、生物信息学、特殊数据处理应用等方向，能够解决未见胃癌诊断和预警的指纹图谱模型等问题，达到操作简单、资源丰富的效果

Active Publication Date: 2014-07-02
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the search, there is no report closely related to the detection of trace volatile organic compounds in the metabolites of gastric cancer cells, and there is no fingerprint model for the diagnosis and early warning of early gastric cancer

Method used

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  • Compound fingerprint atlas model used in early-stage gastric cancer diagnosis/early warning, and establishing method thereof
  • Compound fingerprint atlas model used in early-stage gastric cancer diagnosis/early warning, and establishing method thereof
  • Compound fingerprint atlas model used in early-stage gastric cancer diagnosis/early warning, and establishing method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] like image 3 As shown, take the cells to be tested, and detect the concentration of 4-isopropoxybutanol (Peak5), nonanal (peak6) and 4-butoxyn-butanol (peak9) in the volatile metabolites, and detect The result is compared with the organic compound fingerprint model of the present invention, 4-isopropoxybutanol [gastric cancer cell] / [normal gastric mucosal cell]≤0.31, nonanal [gastric cancer cell] / [normal gastric mucosal cell] ≤0.36, 4-butoxy n-butanol [gastric cancer cells] / [normal gastric mucosal cells] ≤0.40, can be initially screened for early gastric cancer.

Embodiment 2

[0039] like Figure 4 As shown, take the subject’s cells to be tested, detect the volatile metabolites therein, compare the detection results with the organic compound fingerprint model of the present invention, and analyze according to the process shown in the model: 4-isopropyl Oxybutanol[gastric cancer cells] / [normal gastric mucosal cells]≤0.31, nonanal[gastric cancer cells] / [normal gastric mucosal cells]≤0.36, 4-butoxy n-butanol[gastric cancer cells] / [normal gastric Mucosal cells] ≤ 0.40, can be initially screened for early gastric cancer. It was further detected that there were characteristic peaks in volatile organic metabolites: 3-octanone (peak2), 2-butanone (peak8). Then the effect of prompting early gastric cancer can be further strengthened.

[0040] The present invention uses the above-mentioned model to perform early warning of gastric cancer at the cell level in vitro. The fingerprint model is used in the detection of melanoma cells, lung cancer cells, gastric...

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Abstract

Volatile organic compounds emitted from gastric cancer cell metabolite is separated and detected using HS-SPME / GC-MS. A volatile compounds fingerprint atlas-spectrum model are disclosed for early gastric cancer diagnosis / warning Volatiles 3-octanone and 2-butanone as indicators of gastric cancer cells which are not contained in the headspace of gastric mucosal cells GES-1. Meanwhile, the ratio of mass to volume to concentration of 4-isopropoxy alcohol, nonanoic acid, and 4-butoxy n-butanol is as follows: 4-isopropoxy alcohol [gastric cancer cells] / [normal gastric mucosa cells]≰0.31; nonanoic acid [gastric cancer cells] / [normal gastric mucosa cells]≰0.36; and 4-butoxy n-butanol [gastric cancer cells] / [normal gastric mucosa cells]≰0.40. The volatile organic compound in cell metabolite to be detected is compared with the fingerprint atlas-spectrum model, so as to implement screening and warning of the early gastric cancer.

Description

technical field [0001] The invention relates to a compound fingerprint model and establishment method for early gastric cancer diagnosis / early warning, providing help for early warning of gastric cancer. Background technique [0002] The incidence of gastric cancer ranks second among malignant tumors in my country, and its case fatality rate ranks first. In recent years, the proportion of young patients has been increasing year by year. At present, the detection of gastric cancer is mainly based on one or more methods such as X-ray barium meal method, fiber optic endoscopy (including endoscopic ultrasonography), histopathological examination, and serum tumor markers. The radiation and medicines taken by the patient during the examination process of the above methods bring certain side effects, and the applicable objects are limited. Moreover, these methods are based on tumor size to diagnose gastric cancer, and the confirmation rate for early or microscopic gastric cancer ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N2030/8831G01N30/8686G01N33/5091G06F19/12G16B5/00
Inventor 张益霞崔大祥
Owner SHANGHAI JIAOTONG UNIV
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