Liquid nitrogen medium/low-temperature crusher for biological sample

Abstract

The invention belongs to the field of biomedical instruments and relates to a liquid nitrogen medium/low-temperature crusher for a biological sample. The liquid nitrogen medium/low-temperature crusher comprises a bottom plate, a limiting fork, a stand column, a lever, a sample cup, sample cup cover, a sample pressure head, a horseshoe-shaped cushion block and a pressurizing slider. A use method of the liquid nitrogen medium/low-temperature crusher comprises the steps of: placing the biological sample at the bottom of the sample cup, pouring liquid nitrogen, covering the sample cup cover, placing the sample pressure head into a hole in the center of the sample cup cover, meanwhile, placing the horseshoe-shaped cushion block so as to erect a circular disc shaped structure on the upper end of the sample pressure head on the horseshoe-shaped cushion block, suspending the sample pressure head in the liquid nitrogen to decrease the temperature of the sample pressure head, removing the horseshoe-shaped cushion block after the temperature of the sample pressure head is decreased, lowering the sample pressure head onto the biological sample, moving the sample cup, the sample cup cover and the sample pressure head to lean against the limiting fork on the bottom plate, and forcibly pressing the lever to transfer the pressure of the lever by the pressurizing slider on the lever to the biological sample in contact with the lower end of the sample pressure head via the sample pressure head, thereby crushing the low-temperature solid-state biological sample in the liquid nitrogen.

Description

Technical field: [0001] The invention belongs to the field of biomedical instruments, and relates to a low-temperature crusher in liquid nitrogen for biological samples, which functions to crush biological tissues at a low temperature of -196°C in liquid nitrogen. Background technique: [0002] The current crushing method of biological tissue is as follows. [0003] For soft tissues such as muscle, liver, spleen, etc., use a scalpel or scissors to directly divide them into small tissue pieces, and then use a homogenizer to break the tissue. [0004] The bone tissue was divided into small pieces with a rongeur and could not be further broken with a homogenizer because the bone tissue was too hard. The current method is to transfer the bone tissue block into a mortar filled with liquid nitrogen. The bone tissue is hard and brittle under the low temperature of liquid nitrogen. The bone tissue is ground and pressed with a grinding rod, and the bone tissue is ground into a powde...

AI Technical Summary

Problems solved by technology

In short, this method is labor-intensive, has sample loss, is not very fine bone tissue mixed in the ground powder, takes a long time to grind, and the grinding effect is also related to the experience of the grinder.

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