The invention belongs to the field of biomedical instruments and relates to a liquid nitrogen medium/low-temperature crusher for a biological sample. The liquid nitrogen medium/low-temperature crusher comprises a bottom plate, a limiting fork, a stand column, a lever, a sample cup, sample cup cover, a sample pressure head, a horseshoe-shaped cushion block and a pressurizing slider. A use method of the liquid nitrogen medium/low-temperature crusher comprises the steps of: placing the biological sample at the bottom of the sample cup, pouring liquid nitrogen, covering the sample cup cover, placing the sample pressure head into a hole in the center of the sample cup cover, meanwhile, placing the horseshoe-shaped cushion block so as to erect a circular disc shaped structure on the upper end of the sample pressure head on the horseshoe-shaped cushion block, suspending the sample pressure head in the liquid nitrogen to decrease the temperature of the sample pressure head, removing the horseshoe-shaped cushion block after the temperature of the sample pressure head is decreased, lowering the sample pressure head onto the biological sample, moving the sample cup, the sample cup cover and the sample pressure head to lean against the limiting fork on the bottom plate, and forcibly pressing the lever to transfer the pressure of the lever by the pressurizing slider on the lever to the biological sample in contact with the lower end of the sample pressure head via the sample pressure head, thereby crushing the low-temperature solid-state biological sample in the liquid nitrogen.