Method for detecting content of trehalose in artemia eggs

A detection method and trehalose technology, applied in the field of biochemistry, can solve the problem of not being efficient and economical, and achieve the effects of complete crushing, short crushing time and obvious color development effect.

Active Publication Date: 2010-08-04
QILU UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] At present, the qualitative and quantitative analysis of trehalose mainly relies on high-performance liquid chromatography in foreign countries. However, when high-performance liquid chromatography is not yet common in my country, the application of this method is often limited by laboratory conditions. And the rapid qualitative and quantitative analysis of a large amount of trehalose in the extraction process, completely relying on high performance liquid chromatography is not an efficient and economical method, but it is very convenient to use thin layer chromatography for qualitative and quantitative analysis of trehalose

Method used

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  • Method for detecting content of trehalose in artemia eggs
  • Method for detecting content of trehalose in artemia eggs
  • Method for detecting content of trehalose in artemia eggs

Examples

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Effect test

Embodiment 1

[0060] Weigh 5 g of the Artemia egg product and make a 400 mL solution with distilled water. Ultrasonic cell disruptor was used for processing, and the crushing conditions were power 200W, working time 10s, intermittent time 10s, and the total process was 30min. The crushed Artemia cell solution was centrifuged at 10000r / min for 10min, and the supernatant was selected for sampling. When applying samples, select the trehalose standard sample concentration as 1mg / ml, and the sample application volume is 2ul; the sample application volume of the Artemia sample is 2ul.

[0061] Select the developer as n-butanol:pyridine:water at a volume ratio of 4:3:1, mix 20ml, put it into the chromatographic tank for chromatography, and select the color developer as sulfuric acid:methanol at a volume ratio of 1:4, mix 25ml , sprayed on the chromatographic plate with a spray device. Put it in an oven and dry it at 106°C for 30 minutes to develop the color. Such as Figure 4 shown. On the le...

Embodiment 2

[0063] Weigh 1 g of Artemia egg product and make a 100 ml solution with distilled water. Ultrasonic cell disruptor was used for processing, and the crushing conditions were power 300W, working time 5s, intermittent time 5s, and the total process was 20min. The crushed Artemia cell solution was centrifuged at 5000r / min for 20min, and the supernatant was selected for sampling. When applying samples, select the trehalose standard sample concentration as 1mg / ml, and the sample application volume is 2ul; the sample application volume of the Artemia sample is 2ul.

[0064] Select the developer as n-butanol:pyridine:water at a volume ratio of 4:3:1, mix 20ml, put it into the chromatographic tank for chromatography, and select the color developer as sulfuric acid:methanol at a volume ratio of 1:4, mix 25ml , sprayed on the chromatographic plate with a spray device. Put it in an oven and dry it at 106°C for 30 minutes to develop the color. Such as Figure 5 shown. The left side is...

Embodiment 3

[0066] Weigh 0.5 g of Artemia egg product, and make a 40 ml solution with distilled water. Ultrasonic cell disruptor was used for processing, and the crushing conditions were power 250W, working time 8s, intermittent time 8s, and the total process was 25min. The crushed Artemia cell solution was centrifuged at 8000r / min for 15min, and the supernatant was selected for sampling. When applying samples, choose trehalose standard sample concentration as 1mg / ml, sample application volume 4ul; Artemia sample application volume 3ul.

[0067] Select the developer as n-butanol:pyridine:water at a volume ratio of 4:3:1, mix 20ml, put it into the chromatographic tank for chromatography, and select the color developer as sulfuric acid:methanol at a volume ratio of 1:4, mix 25ml , sprayed on the chromatographic plate with a spray device. Put it in an oven and dry it at 106°C for 30 minutes to develop the color. Such as Image 6 shown. The left side is the trehalose standard, and the ri...

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Abstract

The invention relates to a method for detecting the content of trehalose, in particular to a method for detecting the content of the trehalose in artemia eggs, which belongs to the technical field of biochemistry. The method for detecting the content of the trehalose in the artemia eggs comprises the following steps: crushing cells by ultrasonication; treating the crushed cells; and detecting by a thin-layer chromatography. The detecting method of the invention utilizes ultrasonication to crush artemia egg cells, and detecting the content of the trehalose by the thin-layer chromatography qualitatively and quantitatively. Compared with the traditional method, the method for detecting the content of the trehalose in the artemia eggs has the advantages of simple pretreatment, audio-visual and obvious results, rapid and convenient detection and the like.

Description

technical field [0001] The invention relates to a method for detecting the content of trehalose, in particular to a method for detecting the content of trehalose in Artemia eggs, and belongs to the technical field of biochemistry. Background technique [0002] Artemia belongs to Crustacea, Branchiopoda, Anostraca, Artemidae, and is an economically important aquatic crustacean, distributed in almost all salt pans around the world. and hypersaline lakes. Regardless of hermaphroditism or parthenogenesis, Artemia has two production methods, one is oviparous and the other is oviparous. The diapausing eggs produced by oviparous methods are called dormant eggs. The dormant eggs are embryos containing 3,000 to 4,000 cells, which are covered with complex embryonic membranes and shells, which can resist various adverse environments and can be stored for a long time in a dry state. Under suitable water and temperature, metabolism can be restored and hatched into nauplii. The formati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N1/28
Inventor 王瑞明马春玲王腾飞李丕武徐汝意
Owner QILU UNIV OF TECH
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