Ammonia (ammonia ion) determination method and ammonia (ammonia ion) diagnosis/determination kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food inspection and determination, and can solve problems such as undiscovered blood ammonia determination methods
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Embodiment 1
[0133] Embodiment 1: the mensuration of ammonia (ammonia ion) content in blood plasma
[0134] 1) Preparation of standard samples
[0135] Dissolve a certain amount of ammonia salt in water or buffer solution, and then adjust the ammonia concentration to 100 micromole / liter;
[0136] 2) Pretreatment of samples to be tested
[0137] Plasma samples are used as samples to be tested without pretreatment;
[0138] 3) Blank sample
[0139] Described water or buffer solution are used as blank sample, and its ammonia concentration is 0 micromole / liter;
[0140] B. Preparation of reagent solution:
[0141] The ammonia diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:
[0142] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0143] Glycerin 1mol / L
[0144] NADP + 0.25mmol / L
[0145] Ammonia kinase 16000U / L
[0146] Pyruvate carboxylase 18000U / L
[0147] Fatty acyl-CoA synthetase 12...
Embodiment 2
[0165] Embodiment 2: the mensuration of ammonia (ammonia ion) content in blood plasma
[0166] 1) Preparation of standard samples
[0167] A certain amount of ammonia salt is dissolved in water, and then the concentration of ammonia (ammonia ion) is adjusted to 100 micromole / liter, as a standard sample;
[0168] 2) Preparation of samples to be tested
[0169] Plasma samples are used as samples to be tested without pretreatment;
[0170] 3) Blank sample
[0171] Described water is used as blank sample, and its ammonia (ammonia ion) concentration is 0 micromole / liter;
[0172] B. Preparation of reagent solution:
[0173] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:
[0174] Reagent 1 with the following composition:
[0175] Phosphate buffer 100mmol / L
[0176] NAD + 0.25mmol / L
[0177] Adenosine triphosphate 5mmol / L
[0178] Oxaloacetate 5mmol / L
[0179] Coenzyme A 5mmol / L
[0180] Long chain-acyl-...
Embodiment 3
[0203] Embodiment 3: the mensuration of ammonia (ammonia ion) content in plasma sample
[0204] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:
[0205] B. Preparation of reagent solution:
[0206] Ammonia (ammonia ion) diagnostic / assay reagent is a three-dose reagent that contains:
[0207] Reagent 1 with the following composition:
[0208] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0209] NAD + 0.25mmol / L
[0210] Adenosine triphosphate 6mmol / L
[0211] Oxaloacetate 5mmol / L
[0212] Coenzyme A 5mmol / L
[0213] Long-chain-acyl-CoA 5mmol / L
[0214] Monovalent phosphate 4mmol / L;
[0215] Reagent 2 with the following composition:
[0216] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0217] Sodium chloride 2mol / L
[0218] Pyruvate carboxylase 28000U / L
[0219] Fatty acyl-CoA synthetase 50000U / L;
[0220] Reagent 3 with the following composition:
...
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