Method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice and application of method

Abstract

The invention discloses a method for breeding indica sterile line-japonica restorer line intersubspecific hybrid rice. A hybridization polymerization and testcross screening alternate progressive breeding method is utilized according to a breeding target; a japonica type widely compatible restorer line in which a wide compatibility gene, a male sterile cytoplasmic restorer gene and multiple kinds of functional genes are polymerized is bred by integrating various breeding technologies; when an indica-japonica intersubspecific hybrid rice combination is prepared by using the japonica type widely compatible restorer line, an indica three-line sterile line and an indica two-line sterile line, the problems that first generation of indica-japonica hybrid is low in yield, high in plant, long in growth period and low in grain plumpness can be effectively solved; an effective breeding way and an effective breeding method are provided for breeding indica-japonica hybrid rice which directly utilizes powerful intersubspecific hybrid advantages; and the method is applied in the field of the research on the utilization of intersubspecific hybrid advantages of rice three-line and two-line methods.

Description

technical field [0001] The invention relates to the field of rice hybrid breeding, in particular to a method for breeding hybrid rice between indica and non-japonica subspecies. [0002] The method of the invention is applicable to the selection and breeding of the hybrid rice between indica and japonica subspecies by the three-line method and the two-line method. Background technique [0003] At present, the annual promotion area of ​​hybrid rice in my country is more than 13 million hectares, but almost all hybrid rice combinations belong to inter-variety hybrid rice. It has become the consensus of rice breeders to make more or direct and comprehensive use of heterosis among rice subspecies in order to further increase yield. It is very difficult technically to breed a combination of indica-japonica inter-subspecific hybrid rice with strong advantages that can be used practically. The main problem is that the first generation of indica-japonica hybrids has low seed sett...

AI Technical Summary

Problems solved by technology

It is very difficult technically to breed a combination of indica and japonica inter-subspecific hybrid rice with strong advantages that can be used practically. Such a difficult problem, the direct use of heterosis between rice subspecies has not achieved breakthroughs for a long time

[0004] There are two types of hybrid rice, the three-line method and the two-line method, each with its own advantages and disadvantages: the two-line method has the three advantages of free combination, simple seed production procedure, and avoiding the homogeneity of sterile cytoplasm, but due to the light (temperature) sensitive nuclear The reproduction and seed production of sterile lines are restricted by time and space conditions such as light and temperature, and there are uncertain risk factors. The technical requirements for reproduction and seed production are higher than those of the three-line method

However, in the process of breeding practical hybrid rice between indica and non-japonica subspecies, it is found that there are still many problems to be solved

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