Method for identifying cordyceps sinensis through microscopic staining

A technology for Cordyceps sinensis powder and Cordyceps sinensis, which is applied in the field of medicinal material identification, can solve problems such as inability to detect impurities, difficulty in distinguishing authenticity, inability to identify counterfeit products, etc., and achieves the effects of protecting interests, not easy to interfere, and low cost

Active Publication Date: 2012-07-18
QINGHAI SPRING MEDICINAL RESOURCES TECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The pure Cordyceps sinensis powder that is currently circulated and sold in the market is made into a certain dosage form, so it is difficult to distinguish the authenticity from the fake, although the means of testing equipment can be used to prove the existence of s

Method used

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  • Method for identifying cordyceps sinensis through microscopic staining
  • Method for identifying cordyceps sinensis through microscopic staining
  • Method for identifying cordyceps sinensis through microscopic staining

Examples

Experimental program
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Embodiment Construction

[0033] 5. Experimental methods and parameters:

[0034] 1. Experimental samples:

[0035] Cordyceps slices, commercially available Jicao brand pure Cordyceps superfine powder, starch superfine powder, starch superfine powder and pure Cordyceps superfine powder mixture.

[0036] 2. Dyeing agent

[0037] Fast green dyeing solution: Sinopharm Group Chemical Reagent Co., Ltd.; safranin dyeing solution: Sinopharm Group Chemical Reagent Co., Ltd.; eosin dyeing solution: Shenyang Xinguang Chemical Factory; hematoxylin dyeing solution: Shenyang Xinguang Chemical Factory; Steiner's solution: Beijing Chemical plant; iodine: Nanjing Xinhuayuan Chemical Co., Ltd., prepared dye solution according to conventional methods.

[0038] 3. Instruments or other utensils

[0039] Binocular biological microscope, camera adapter, Canon SLR camera. Alcohol lamps, glass slides, coverslips, staining racks, cedar oil, absolute ethanol, xylene, Canadian gum, lens cleaning paper, distilled water and ot...

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Abstract

The invention belongs to the field of crude medicine identification, and particularly relates to a method for identifying cordyceps sinensis through microscopic staining. The invention aims to solve the technical problem that the authenticity of the cordyceps sinensis powder which is circulated and sold in the market is difficult to identify. The technical scheme for solving the problem is to provide the method for accurately identifying the cordyceps sinensis powder. The method comprises the following steps that: the cordyceps sinensis powder to be inspected is taken, stained and produced into a smear; and the produced smear is subjected to microscopic observation under a microscope, if the special structure of the cordyceps sinensis of each smear can be clearly observed, the obvious blue spots under the iodine staining are not existed, and the obvious plant tissues are not observed, the cordyceps sinensis powder can be identified to be pure, otherwise, the cordyceps sinensis powder is not pure. The method can clearly identify the fine medicinal slice powder and the matter which does not belong to the cordyceps sinensis, is low in cost, has high efficiency, is not easy to be disturbed by various addition ingredients, and has very high accuracy and a good application prospect in the field.

Description

[0001] This application is a divisional application of an invention patent application with an application date of April 21, 2010, an application number of 201010151881.9, and an invention title of "Method for Differentiating Winter, Spring and Summer Grass Powder by Microscopic Staining". technical field [0002] The invention belongs to the field of medicinal material identification, and in particular relates to a method for identifying cordyceps sinensis powder by microscopic staining. Background technique [0003] In the traditional medicine of our country, Cordyceps sinensis has always been a high-grade tonic, and its medicinal status is very prominent. It is also known as the "three major tonics" together with ginseng and velvet antler. With the in-depth research in various related disciplines, there have been reports of new species and new distributions of Cordyceps in recent years. Confirmed the efficacy of Cordyceps sinensis. Due to the special growth environment, ...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N21/84
Inventor 张雪峰徐丽
Owner QINGHAI SPRING MEDICINAL RESOURCES TECHNOLOGY CO LTD
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