Application of teprenone in resisting neurotoxicity caused by methamphetamine

A technology of methamphetamine and teprenone, which is applied in the field of medicine to achieve the effect of high safety, significant curative effect and low cost

Inactive Publication Date: 2012-07-25
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether teprenone protects against ampheta

Method used

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  • Application of teprenone in resisting neurotoxicity caused by methamphetamine
  • Application of teprenone in resisting neurotoxicity caused by methamphetamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Determination of the protective effect of teprenone on methamphetamine-stimulated PC12 cells by tetrathiazolium blue (MTT) method

[0021] 1. Culture of PC12 cell line

[0022] PC12 cells were cultured in RPMI1640 medium containing 10% horse serum, 5% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin at 37°C, 5% CO 2 and 95% humidity for adherent culture. Replace the medium every 1-2 days, and when the cells reach about 70%-80% confluence, passage or use for experiments.

[0023] 2. Arrangement of PC12 cells

[0024] The adherent PC12 cells were digested with 0.25% trypsin and centrifuged at 1500rpm for 5min to remove trypsin. Cells were evenly arranged in a 96-well plate with a density of 1×10 4 Cell / well, in RPMI1640 medium containing 10% horse serum, 5% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin, at 37°C, 5% CO 2 and 95% humidity for adherent culture.

[0025] 3. Stimulate PC12 cells in groups

[0026] The ce...

Embodiment 2

[0034] Example 2: Western Blot detection teprenone restores methamphetamine-induced reduction of pro-caspase3 protein

[0035] 1. Cell line culture and drug stimulation

[0036] PC12 cells were cultured in 1640 medium containing 10% horse serum, 5% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin at 37°C, 5% CO 2 Adhesive culture is carried out at 95% humidity, and the culture medium is replaced every 2-3 days. When the cells reach about 70%-80% confluency, they are passaged or used for experiments.

[0037] The cells were seeded in six-well plates, and after overnight culture, the cells were treated with drugs. The groups were treated as follows: the control group did not receive drugs, and the experimental group received: (1) 2 mM methamphetamine; (2) 10 μM teprenone pre-stimulated 4 hours in advance, and then added 2 mM methamphetamine; ( 3) 10 μM teprenone alone was pre-stimulated 4 hours in advance. After the above treatments, place at 37 °C and 5% CO...

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Abstract

The invention discloses an application of teprenone in the preparation of drugs for preventing and/or treating neurotoxicity caused by methamphetamine, belonging to the technical field of medicines. The teprenone has the effect of resisting neurotoxicity caused by methamphetamine. A test result shows that the teprenone has the capabilities of protecting rat pheochromocytoma cells, namely PC12 cells to be free from cell apoptosis caused by methamphetamine and recovering the pro-caspase3 protein reduction caused by methamphetamine, and has the advantages of obvious treatment effect, high safety, low cost and suitability for practical popularization and application.

Description

technical field [0001] The invention relates to a new medical application of teprenone, in particular to the application of teprenone in the preparation of drugs for preventing and / or treating neurotoxicity caused by methamphetamine, and belongs to the technical field of medicine. Background technique [0002] The abuse of amphetamines is one of the social hazards in the world today. Common amphetamines include amphetamine, methamphetamine (MA), ephedrine, methylenedioxymethylamphetamine (methylenedioxymethylamphetamine, MDMA), etc. Amphetamines are similar to catecholamine neurotransmitters, and have significant central excitatory and peripheral α, β adrenergic receptor excitatory effects, which can constrict peripheral blood vessels, excite the heart, increase blood pressure, relax bronchial smooth muscle, dilate pupils, and contract bladder sphincter, etc. effect. The main targets of amphetamines are dopaminergic neurons, and their toxic mechanisms include dopamine oxid...

Claims

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Application Information

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IPC IPC(8): A61K31/121A61P25/30
Inventor 白洁吕涛魏大巧
Owner KUNMING UNIV OF SCI & TECH
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