Method for extracting leaf of Chinese ilex of broadleaf holly leaf, total saponin and application thereof
Active Publication Date: 2012-07-25
PEKING UNIV
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There are many varieties of Kudingcha on the market. According to investigations, the leaves of 22 kinds of pla
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[0029] Example 1
[0030] This example is used to illustrate the extraction method of Ilex Kudingcha leaves provided by the present invention.
[0031] Extraction: Weigh 0.5Kg of Ilex kudingcha leaf medicinal material, extract with 5L and 4L of 60% ethanol respectively and filter twice, the reflux time is 0.5 hour and 1 hour respectively, and the filtrate is combined;
[0032] In addition to ethanol: Distill the filtrate obtained by extraction under reduced pressure until it has no alcohol taste, and recover the ethanol. The relative density of the concentrated solution is 1.06~1.08 (room temperature);
[0033] Adsorption: Adsorb the obtained solution on a HPD400A macroporous resin column, the filling amount of the resin column is 5Kg, the diameter-to-height ratio is 1:4, the sample is repeated 3 times, and the static adsorption is 30 minutes;
[0034] Wash impurities: first rinse the resin column with 4 column volumes of deionized water until sugar-free; then rinse with 8 column volum...
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[0037] Example 2
[0038] This example is used to illustrate the extraction method of Ilex Kudingcha leaves provided by the present invention.
[0039] Extraction: Weigh 0.5Kg of Ilex kudingcha medicinal material, and use 4L of 65% ethanol, 3L of 60% ethanol and 3L of 50% ethanol to extract and filter for three times, the reflux time is 2 hours and 1 hour respectively And 0.5 hours, combine the filtrate;
[0040] In addition to ethanol: Distill the filtrate obtained by extraction under reduced pressure until it has no alcohol taste, and recover the ethanol. The relative density of the concentrated solution is 1.06~1.08 (room temperature);
[0041] Adsorption: adsorb the obtained solution on an AB-8 macroporous resin column, the filling amount of the resin column is 3.5Kg, and the diameter-to-height ratio is 1:8;
[0042] Wash impurities: first rinse the resin column with 8 column volumes of deionized water until sugar-free; then rinse with 10 column volumes of 30% alkaline ethanol (Na...
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Abstract
The invention provides a method for extracting leaf of Chinese ilex of broadleaf holly leaf, and total saponin of the leaf of Chinese ilex of the broadleaf holly leaf extracted by the method and application thereof. The method comprises the following steps of: (1) extracting the leaf of Chinese ilex of the broadleaf holly leaf by using 50-70% ethanol aqueous solution in a backflow manner;(2) filtering and removing ethanol in an extracting solution; (3) adsorbing a large-hole resin column in the solution obtained by the step (2), and eluting the large-hole resin column sequentially by using water, 10-30% alkaline ethanol aqueous solution with a pH value of 9-11, 10-30% ethanol aqueous solution and 50-70% ethanol aqueous solution; and (4) eluting the large-hole resin column by using the 50-70% ethanol aqueous solution to obtain a solution, and concentrating and drying the solution to obtain the leaf of Chinese ilex of the broadleaf holly leaf. The prepared total saponin can be applied to preparing medicines for reducing cholesterol and blood fat and resisting atherosclerosis.
Description
technical field [0001] The invention relates to a method for extracting kudingcha holly leaves, total saponins of kudingcha holly leaves extracted by the method and uses thereof. Specifically, the present invention relates to a method for extracting the total saponins of the leaves of Kudingcha holly. The total saponins extracted by the method can be used for lowering cholesterol, lowering blood fat, and resisting atherosclerosis. Background technique [0002] Hyperlipidemia includes high cholesterol and high triglycerides, and the former is far more harmful than the latter. For hyperlipidemia, drug-induced lipid-lowering therapy is the most effective measure to treat hyperlipidemia. At present, there are many effective drugs for lowering triglycerides, but the effective drugs for lowering cholesterol are mainly statins. This type of drug is mainly used to fight arteriosclerosis caused by hypercholesterolemia, and its mechanism of action is to inhibit the synthesis of chol...
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