The invention relates to a special clinical nutrient composition for gestational diabetes and a preparation method of the nutrient composition. The composition comprises 20-35 parts of protein, 8-15 parts of fat, 25-40 parts of carbohydrate, 3-8 parts of dietary fiber, 0.6-1.2 parts of macroelements, 0.01-0.03 parts of microelements, 0.005-0.015 parts of fat-soluble vitamins, 0.08-0.2 parts of water-soluble vitamins, 0.05-0.6 parts of dietary essence, 0.5-4 parts of medicinal and edible components, 0.1-1 part of natural plant compounds and 0.1-0.4 parts of new resource food, wherein the medicinal and edible components are selected from at least one of Chinese yams, black sesame, radix puerariae, rhizoma polygonate, folium mori, fructus lycii, radix platycodonis and lilies; powder is prepared from the components in advance, and grease powder is prepared from fat with a microencapsulating technology. Through sieving and proportioning of the components, the nutrient composition can provide comprehensive nutrition for patients suffering from gestational diabetes, and digestion and absorption are facilitated; besides, the components with the blood glucose reducing function are added to assist in reducing the blood glucose level of the gestational diabetes and the disease risk, and growth demands and normal development of fetuses are guaranteed.