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Method for improving foaming property and foam stability of serum albumin by utilizing chitosan oligosaccharide

A technology for serum albumin and foam stability, which can be used in animal protein processing, application, pharmaceutical formulations, etc., and can solve problems such as complex processes

Active Publication Date: 2020-07-07
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The modification process needs to be reacted at a certain temperature and pH for several hours, and at the same time, high temperature is required to inactivate the enzyme at the end. The whole process is relatively complicated.

Method used

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  • Method for improving foaming property and foam stability of serum albumin by utilizing chitosan oligosaccharide
  • Method for improving foaming property and foam stability of serum albumin by utilizing chitosan oligosaccharide
  • Method for improving foaming property and foam stability of serum albumin by utilizing chitosan oligosaccharide

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Embodiment 1

[0027] The present embodiment provides a kind of method utilizing chitosan oligosaccharide to improve foamability and foam stability of serum albumin, comprising the steps:

[0028] Step 1: Weigh a certain amount of bovine serum albumin powder and dissolve it in deionized water to prepare a bovine serum albumin solution with a concentration of 2%;

[0029] Step 2: Weigh a certain amount of chitosan oligosaccharide powder and dissolve it in deionized water to prepare a 4% chitosan oligosaccharide solution;

[0030] Step 3: Mix the bovine serum albumin solution and chitosan oligosaccharide solution in step 1 and step 2 to prepare a mixed system with chitosan oligosaccharide / bovine serum albumin ratio of 1:4, and fix the quality of bovine serum albumin in the system The concentration is 1%, mixed at 300 rpm for 15 min, adjusted to pH 5.0 with 1M hydrochloric acid and sodium hydroxide, and continuously stirred at 300 rpm for 30 min to obtain chitooligosaccharide / bovine serum album...

Embodiment 2- Embodiment 4

[0032] Embodiment 2-Example 4 except that the mass ratio of chitosan oligosaccharide and serum albumin is different, all the other are the same as embodiment 1, specifically see table 1.

Embodiment 5

[0034] This embodiment is changed into 7.0 except that pH, all the other are the same as embodiment 1, see table 1 specifically.

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Abstract

The invention discloses a method for improving the foaming property and foam stability of serum albumin by utilizing chitosan oligosaccharide. The method comprises the following steps: adding chitosanoligosaccharide into serum albumin, performing uniform mixing in a solvent, and regulating the pH of the mixture to be 5-9. The invention further provides a foaming agent. The foaming agent is a serum albumin / chitosan oligosaccharide system, namely a mixed solution with the pH of 5-9 prepared by dissolving serum albumin and chitosan oligosaccharide in the solvent. According to the method for improving the foaming property and foaming stability of serum albumin by utilizing chitosan oligosaccharide, the noncovalent static interaction can be generated from chitosan oligosaccharide and serum albumin by adjusting the pH value, a chitosan oligosaccharide / serum albumin compound is formed, and the foaming property and foam stability of the serum albumin can be remarkably improved with simple combination. The method is simple in process and can reduce time cost.

Description

technical field [0001] The invention relates to the technical field of protein foaming, in particular to a method for improving the foaming properties and foam stability of serum albumin by utilizing chitosan oligosaccharides. Background technique [0002] Serum albumin is the most abundant protein in plasma, accounting for 60% of plasma protein content, and is a macromolecule with important physiological functions in organisms. Serum albumin has good water solubility, convenient preparation, high stability and rich nutritional value. It is mainly used in food, medicine, animal feed, cell culture and other fields. Blood tofu with serum albumin as the main component is also a very Foods that are popular with consumers. Oligochitosan is a degradation product of chitosan or chitin. It has biological activities such as reducing blood fat, anti-oxidation, antibacterial, accelerating calcium and iron absorption, and inhibiting tumor cell growth. It has good thickening and water-h...

Claims

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Application Information

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IPC IPC(8): A61K31/722A61K47/42A61K47/36A61P3/06A61P39/06A61P31/04A61P31/10A61P3/02A61P35/00A23J3/12A23L29/30A23L29/00
CPCA23J3/12A23V2002/00A61K31/722A61K47/36A61K47/42A23L29/035A23L29/30A61P3/02A61P3/06A61P31/04A61P31/10A61P35/00A61P39/06A23V2200/226A23V2250/511A23V2250/5436
Inventor 赵萌张慧
Owner HUBEI UNIV OF TECH
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