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Composite lactobacillus inoculant and application thereof in buffalo silage

A technology for compounding milk and Lactobacillus fermentum, applied in the field of microorganisms, can solve the problems of inability to enrich probiotics, deterioration of silage quality, poor quality of roughage, etc., and achieves the promotion of gastrointestinal mucosal healing, reduction of intestinal barrier permeability, The effect of good fermentation bacteria environment

Active Publication Date: 2020-04-21
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nowadays, under the background of the rapid development of my country's animal husbandry, individual breeding is also growing stronger. However, in the ruminant breeding industry, due to cost constraints, many self-employed do not add microorganisms and only use waste materials to prepare silage. This method is simple. , extensive, but the quality of the silage obtained is not good, and if the control of the flora is not proper, the probiotics cannot be enriched to form dominant bacteria, which can effectively inhibit the growth of miscellaneous bacteria
In the individual extensive breeding process, due to improper operation, oxygen enters the surface of the silo, which will face the problem of secondary fermentation of the silage, that is to say, a large number of harmful microorganisms begin to recover, including yeast and mold spores, which are carried out together with acetic acid Aerobic spoilage, leading to poor overall silage quality
These microorganisms use oxygen to start a large number of reproduction, which will cause the temperature in the silage to rise, the lactic acid will decompose, the pH value will rise, and the nutritional value will decrease, which will eventually lead to the lack of roughage and poor quality. Therefore, the development of silage lactic acid bacteria additives is currently the easiest way to solve the above problems. An effective method, through the optimization of strains, improving the dominance of lactic acid bacteria in silage can achieve the purpose of improving the fermentation quality of silage

Method used

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  • Composite lactobacillus inoculant and application thereof in buffalo silage
  • Composite lactobacillus inoculant and application thereof in buffalo silage
  • Composite lactobacillus inoculant and application thereof in buffalo silage

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The compound lactobacillus inoculum of this embodiment includes 4 strains of lactobacillus preserved in China, Wuhan, Wuhan University, and China Type Culture Collection Center on August 19, 2019: Lactobacillus fermmentum xf 09#, preserved The number is M2019639; Lactobacillus mucosae xf 13#, the preservation number is M2019640; Lactobacillus acidophilus xf 32#, the preservation number is M2019641; plant Lactobacillus (Lactobacillus plantarum) xf 42#, the preservation number for M2019642.

[0033] 1. Materials and reagents:

[0034] Fermented feed sample: It was taken from an individual milk buffalo breeding farm in Lingshan, Guangxi. The mixed sample was packaged in a sterile Wahaha pure water bottle, stored at room temperature, and delivered to the laboratory on the same day.

[0035] MRS medium (improved MRS medium basis): Guangdong Huankai Microbial Technology Co., Ltd.; M17 solid medium: Qingdao Rishui Biotechnology Co., Ltd.; MRS broth medium: Guangdong Kaihuan M...

Embodiment 2

[0060] Subsequent examples are all based on the strains screened and preserved in Example 1 to prepare bacterial powder. In order to simplify the description, the above-mentioned preserved microorganism Lactobacillus fermentum (Lactobacillus fermmentum) xf 09#, referred to as SL9; Lactobacillus mucosae (Lactobacillus mucosae) xf 13#, referred to as SL13; Lactobacillus acidophilus (Lactobacillus acidophilus) xf 32#, referred to as SL32; Lactobacillus plantarum (Lactobacillus plantarum) xf 42#, referred to as SL42;

[0061] The present embodiment adopts the bacterial strain of embodiment 1 to develop bacterial powder, and specific method is as follows:

[0062] (1) Put a single bacterial species into the liquid concentrated proliferation medium according to the inoculation amount of 10g / kg for fermentation, the fermentation condition is 42°C, anaerobic fermentation, 48h, and the pH is set to 3.92-4.19 according to different bacterial species ( Specifically as shown in table 2) h...

Embodiment 3

[0074] Adopt the bacterium powder that embodiment 2 buffalo milk culture medium prepares to prepare silage, concrete method is:

[0075] The above Lactobacillus is prepared according to the combination of Table 3 (ensilage probiotic strain selection table) to prepare fermented silage. The preparation method is: fresh elephant grass is cut to about 2cm and mixed with brewer's grains according to the mass ratio of 3:7, and then according to Inoculate the lactic acid bacteria agent with an inoculation amount of 10mg / kg, 500g per bag, evacuate and seal with a vacuum sealing machine, store in the dark at room temperature, ferment for 45 days, and measure the corresponding indicators.

[0076] Table 3 Selection of probiotic strains for silage

[0077] group Probiotics group 1 Lactobacillus fermentum xf09#(SL9) group 2 Lactobacillus mucosa xf13# (SL13) group 3 Lactobacillus acidophilus xf32#(SL32) group 4 Lactobacillus plantarum xf42# (SL42) ...

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Abstract

The invention relates to the field of microorganisms, in particular to a composite lactobacillus inoculant and an application thereof in buffalo silage. The composite lactobacillus inoculant is composed of lactobacillus fermentum xf 09#, lactobacillus mucosae xf 13#, lactobacillus acidophilus xf 32# and lactobacillus plantarum xf 42# which are screened and separated from feed raw materials naturally fermented in a peasant household by the applicant. The composite inoculant is homotype lactic acid lactobacillus fermentum, and has the characteristic of high lactic acid yield. The pH value can bequickly lowered in the fermentation process to form a dominant environment for lactobacillus fermentation, the growth of infectious microbes is effectively inhibited, and a favorable fermentation inoculant and environment can provide for silage. Meanwhile, lactobacillus has the effects of bidirectional regulation and diarrhea resistance on intestinal tracts.

Description

【Technical field】 [0001] The invention relates to the field of microorganisms, in particular to a compound lactobacillus agent and its application in buffalo silage. 【Background technique】 [0002] Silage feed is to put the silage raw material into the silage container. Under anaerobic conditions, the lactic acid bacteria attached to the plant convert the soluble sugar into lactic acid. With the increase of lactic acid, when the pH value of the silage drops below 4.2, large The activities of some harmful microorganisms such as spoilage bacteria and butyric acid bacteria are inhibited, thereby preserving the nutritional value of green and juicy feed for a long time. Due to the existence of a large number of lactic acid bacteria and lactic acid, a relatively stable equilibrium state is formed, and the secretion of lactic acid by lactic acid bacteria can maintain The feed will not deteriorate due to the influence of other microorganisms, and at the same time limit the excessive...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18A23K50/10A61K35/747A61K9/19A61P1/00A61P1/12C12R1/225C12R1/23C12R1/25
CPCC12N1/20A23K30/18A23K50/10A61K35/747A61K9/19A61P1/00A61P1/12A23V2400/143A23V2400/113A23V2400/169A61K2300/00
Inventor 谢芳杨承剑谢华德唐振华郭艳霞李孟伟彭开屏
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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