The invention provides an X65 hot-rolled steel plate for a marine riser and a preparation method. The X65 hot-rolled steel plate is prepared from, by weight, 0.03%-0.05% of C, 0.10%-0.30% of Si, 1.10%-1.35% of Mn, smaller than or equal to 0.007% of P, smaller than or equal to 0.0008% of S, 0.01%-0.05% of Alt, 0.04%-0.06% of Nb, 0.008%-0.018% of Ti, 0.20%-0.40% of Ni, 0.15%-0.30% of Cr, 0.10%-0.20%of Mo, smaller than or equal to 0.004% of N and the balance Fe and unavoidable impurity elements. The preparation method comprises the steps that continuous casting blanks with the thickness rangingfrom 300 mm to 500 mm are adopted, differential temperature rolling is adopted for steel blank rough rolling, three-stage rolling is adopted for finish rolling, and then fine and squashed hardened austenite grains are obtained; steel plates are cooled after being rolled, and then fine ferrites and a small amount of acicular ferrite structure are obtained, wherein the initial cooling temperature ranges from 760 DEG C to 780 DEG C, and the final cooling temperature ranges from 200 DEG C to 300 DEG C. The X65 hot-rolled steel plate has the advantages of being large in wall thickness, high in strength, low in yield ratio, excellent in low-temperature toughness, fracture resistance and acid resistance and capable of reducing the fracture and corrosion risks of marine risers.