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Polygene joint detection probe for early diagnosis of tumour, primer and kit

A technology of early diagnosis and combined detection, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of low detection sensitivity, complicated operation, and high cost, and achieve fast detection speed and easy operation. Simple, highly sensitive effects

Inactive Publication Date: 2012-08-08
AMOY DIAGNOSTICS CO LTD
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Problems solved by technology

[0007] At present, the methods for clinical detection of tumor markers mainly include enzyme-linked immunoassay technology and fluorescent immunoassay technology. Early tumor diagnosis can be realized by detecting tumor markers. However, this method is complex in operation, low in detection sensitivity, high in cost, and cannot meet clinical screening requirements. , the actual needs of diagnosis and detection, so that tumor patients miss the best treatment period
Therefore, detection techniques such as ELISA cannot meet the actual needs of clinical applications.

Method used

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  • Polygene joint detection probe for early diagnosis of tumour, primer and kit
  • Polygene joint detection probe for early diagnosis of tumour, primer and kit
  • Polygene joint detection probe for early diagnosis of tumour, primer and kit

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Embodiment 1

[0104] In this example, lung cancer patients are used as the detection objects, and healthy people are used as the control group to further elaborate and verify the detection system of the present invention. The system of the present invention is used to detect P53, KRAS, EGFR, and BRAF in 80 lung cancer patients and 51 healthy people. , PIK3CA and MEK16 gene driver mutations. The method for detecting six gene-driven mutations using the above fluorescent PCR system includes the following steps:

[0105] (1) Screening sample type

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Abstract

The invention discloses a polygene joint detection probe for early diagnosis of tumour, a primer and a kit. The probe and the primer contain SEQ ID NO: 1-SEQ ID NO: 91, can be used for joint detection of p53, KRAS, EGFR, BRAF, PIK3CA and MEK1 genes; can be used to simultaneously detect a plurality of driving abrupt changes in one PCR reaction tube; has high sensitivity and can be used to detect 5-10 copy abrupt change; has strong singularity without generation of non-specificity signals from 10 ng of wild type genome DNA; and has advantages of rapid detection speed, easy operation and low cost.

Description

technical field [0001] The invention relates to biological reagents, in particular to a multi-gene combined detection kit for early diagnosis of tumors. Background technique [0002] Tumor is the most serious disease that endangers human health and is one of the leading causes of human death. According to the data of the World Health Organization (WHO), in 2008, there were approximately 12.7 million new cancer cases and 7.6 million cancer deaths worldwide in 2008. At present, there are about 9 million new cancer patients every year in the world. It is estimated that by 2020, the incidence of cancer worldwide will increase by 50%, and the number of new cancer patients worldwide will reach 15 million every year. The morbidity and mortality rate of cancer in my country ranks first in the world. The number of people who die from cancer every year exceeds 1.6 million, and the incidence of cancer is about 2 million per 100,000 people. One of them died of tumor, ranking first amon...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 阮力张海龙肖桃英江风阁郑立谋
Owner AMOY DIAGNOSTICS CO LTD
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