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Screening method for bentazone sensitive paddy

A technology for bentazon and rice is applied in the field of screening of bentazone-sensitive rice, which can solve the problems of large screening workload, increased cost and time of cultivation, etc., so as to reduce workload, save cultivation cost and cultivation time, and improve the The effect of screening efficiency

Active Publication Date: 2013-07-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the process of cultivating bentazon-sensitive rice, due to the need to harvest all M 2 M on plants 2∶3 Seed or all F 2 F on plants 2∶3 Seeds, and planted by line M 3 or F 3 , and the reserved seeds and the sown seeds must correspond one by one, so this technical route causes a lot of screening work, and the cost and time of cultivation are greatly increased

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Two-line CMS rice Guangzhan 63S

[0035] (1) In June 2010, Guangzhan 63S seed (M 0 generation), half of it was treated with 350Gy dose of γ-rays to obtain M 1 Substitute seeds; the other half were co-treated with 300Gy dose of γ-rays and 0.5% methyl methanesulfonate (MMS) to obtain M 1 generation seeds.

[0036] (2) Put all M 1 The generation seeds were sown in the high-altitude mountain terraces of Yangshan Mountain in Ningguo, Anhui Province, and the mountain spring water was used for string irrigation at the jointing stage to promote M 1 Plants are strong at low temperature, mixed harvest M in October of that year 2 Substitute seeds are about 50kg each.

[0037] (3) In November of the same year, M 2 The seedlings were sown in Lingshui, Hainan, and the seedlings were transplanted to the field as a single plant, with a total of 30 mu; according to the growth of different plants, about 20 days after the earing of the plants (the main ear has turned light...

Embodiment 2

[0040] Example 2 Jiazhe B

[0041] (1) Take the dry seeds of Jiazhe B (M 0 generation) 1500 grams, treated with gamma rays at a dose of 300Gy to obtain M 1 generation seeds.

[0042] (2) Sowing M in Hainan 1 Generation Seed, Spring 2009 M 1 The seed setting rate of the plants decreased, and mixed harvesting of M 2 generation seeds.

[0043] (3) In the early season of 2009, M 2 About 60,000 per plant; according to different plant growth conditions, spray the leaves of the plant with an agricultural sprayer about 15 days after the earing of the plant (the main ear is light yellow, and the grain is in the stage of milk maturity to wax maturity). Mass percentage concentration is the bentazone water agent of 0.5%; In 5-10 days after spraying, observe the growth situation of rice, screen out the plants that appear bentazone poisoning symptoms (leaves appear dehydration green withered, whole plant is dying), altogether 1 plant, recorded as a suspected plant; the seeds on the s...

Embodiment 3

[0048] Example 3 Jiazhe mBx Zixing 101

[0049] (1) Sow bendazol-sensitive rice Jiazhe mB and Bentasone-resistant rice Zixing 101 (ordinary rice) in the field, artificially emasculate Zixing 101 at full flowering stage, and collect Jiazhe mB Ears are crossed and harvested F 1 Seeds; wherein, the plants are bagged and isolated after pollination.

[0050] (2)F 1 Seeds are planted individually and harvested together F 1∶2 Seeds were sown in the field; according to different plant growth conditions, F 2 About 15 days after the plant heading (the main ear has been in light yellow, and the grain is in the milk ripening stage to the wax ripening stage), it is sprayed with an agricultural sprayer with a mass percentage concentration of 0.5% bendazone water; after spraying Within 5-10 days, observe the growth situation of rice, screen out the plants that appear in poisoning symptoms of bendazone (leaves appear to be dehydrated and withered, and the whole plant is dying), and record...

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Abstract

The invention discloses a screening method for bentazone sensitive paddy, which comprises the following steps: sowing paddy seeds; raising seedlings; transplanting; after heading for 15-20 days, spraying a bentazone aqueous solution with mass percent concentration of 0.5%-0.8% to the plants; after 5-10 days, observing the growing condition of the paddy; if the plants are suffered in bentazone poisoned symptom, marking as suspected plants; harvesting seeds on a single suspected plant; taking a part of seeds and sowing; in a seedling stage, spraying the bentazone aqueous solution with mass percent concentration of 0.1%-0.8%; and if the plants are suffered in bentazone lethal symptom after spraying the bentazone aqueous solution, confirming the seeds harvested from the suspected plants as the seeds of the bentazone sensitive paddy. The primary screening for the bentazone sensitive paddy in the seed mature period is the technical core of the screening method provided by the invention. According to the screening method, the seed selection is brought forward for one generation; the sowing area is efficiently reduced; the screening efficiency is greatly increased; the cost is saved; and the screening method is especially suitable for the screening for target plants when the bentazone sensitive paddy is cultivated in a form of mutation breeding or crossbreeding.

Description

technical field [0001] The invention relates to the field of crop cultivation, in particular to a screening method for bendazone-sensitive rice. Background technique [0002] Rice is the grain variety with the largest planting area in China, and has always played a dominant role in my country's grain production and consumption. China is the first country in the world to successfully realize the commercial production of hybrid rice. Since the large-scale promotion and planting of hybrid rice in 1976, the annual planting area of ​​hybrid rice in the country has accounted for more than 50% of the annual planting area of ​​rice. In the past ten years, the photothermosensitive genic male sterile line (T / PGMS) has been successfully utilized, and the two-line hybrid rice has made a breakthrough. So far, many photothermosensitive genic male sterile lines have been identified successively. [0003] The authenticity and purity of hybrid rice varieties are the basis for whether hetero...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G16/00A01G7/06A01G22/22
Inventor 舒庆尧富昊伟王启钊李友发
Owner ZHEJIANG UNIV
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