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Improved method for a highly sensitive detection and quantification of biomolecules using secondary ion mass spectrometry (SIMS)

An ionic and stable technology, applied in biochemical equipment and methods, biomaterial analysis, microbial measurement/inspection, etc., can solve the problem of not allowing quantitative probe/target interaction

Inactive Publication Date: 2012-08-15
BIOSIMS TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method described by Brandt et al suffers from the following disadvantages: (i) it can only be applied to PNA probes or probes that do not contain phosphates and (ii) it does not allow quantification of probe / target interactions

Method used

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  • Improved method for a highly sensitive detection and quantification of biomolecules using secondary ion mass spectrometry (SIMS)
  • Improved method for a highly sensitive detection and quantification of biomolecules using secondary ion mass spectrometry (SIMS)
  • Improved method for a highly sensitive detection and quantification of biomolecules using secondary ion mass spectrometry (SIMS)

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Embodiment

[0191] In the following description, all experiments without a detailed protocol were performed according to standard protocols.

[0192] The following examples are included to illustrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventors to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or a similar result without departing from the spirit and scope of the invention.

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Abstract

The present invention relates to an improved method for detecting and quantifying the presence or absence of a number of biomolecules in a sample using the SIMS technique and arrays for use in said method.

Description

technical field [0001] The present invention relates to methods for the detection and quantification of biomolecules such as DNA, RNA or proteins using isotopic labeling and secondary ion mass spectrometry and arrays designed to carry out the methods. Background technique [0002] In the early 1960s, Castaing and Slodzian developed mass-filtered emission ion microscopy using secondary ions as part of a technique later known as secondary ion mass spectrometry (SIMS). With this technique, a beam of ions (the primary ion beam) is used as a probe to sputter the surface atomic layer of the sample into atoms or clusters of atoms, a small fraction of which is ionized. In a SIMS device, these secondary ions are separated according to mass and then used to measure secondary ion currents to produce, for example, a quantitative atomic mass image of the analyzed surface. [0003] SIMS has become a major tool in semiconductor and surface science research, geochemistry, identification of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N23/225
CPCB01J2219/00527B01J2219/00619B01J2219/00637B01J2219/00702B01J2219/00725C12Q1/02C12Q1/6837C40B20/02C40B20/08G01N33/50G01N33/6848G01N2458/15
Inventor C·里波尔V·诺里斯G·勒让A·德洛纳
Owner BIOSIMS TECH
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