Compound microorganism fungicide for improving water quality of culturing water body and preparation method

A technology of compound microorganisms and water quality improvement, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of water quality over-fertilization and eutrophication, fish floating heads and various diseases

Inactive Publication Date: 2012-08-29
武汉合缘绿色生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention is mainly aimed at problems such as excessive use of chemical fertilizers and organic fertilizers in current aquaculture waters, resulting in excessive wat

Method used

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  • Compound microorganism fungicide for improving water quality of culturing water body and preparation method
  • Compound microorganism fungicide for improving water quality of culturing water body and preparation method
  • Compound microorganism fungicide for improving water quality of culturing water body and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A compound microbial preparation for improving the water quality of aquaculture water, which is made of the following raw materials in mass fraction:

[0036]

[0037] A method for preparing a compound microbial preparation for improving the water quality of aquaculture water, the steps of which are:

[0038] A. The first is the cultivation of Bacillus subtilis and Bacillus licheniformis: Bacillus subtilis and Bacillus licheniformis are cultivated on beef extract peptone medium at 28 or 29 or 30°C for first-level slant culture, and then inoculated into a triangular flask for shaking. Level liquid culture, and finally inoculated on solid medium for third level culture until the number of bacteria reaches 1.0-2.5×10 8 per gram of solid medium.

[0039] B. Followed by the cultivation of yeast: Yeast is cultured on a lactic acid-potato-glucose medium at 28 or 29 or 30°C for primary slope culture, then inoculated into a triangular flask for shaking secondary liquid cultu...

Embodiment 2

[0047] A compound microbial preparation for improving the water quality of aquaculture water, which is made of the following raw materials in mass fraction:

[0048]

[0049] The preparation steps are the same as in Example 1.

Embodiment 3

[0051] A compound microbial preparation for improving the water quality of aquaculture water, which is made of the following raw materials in mass fraction:

[0052]

[0053] The preparation steps are the same as in Example 1.

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Abstract

The invention discloses a compound microorganism fungicide for improving the water quality of a culturing water body and a preparation method. The compound microorganism fungicide is prepared by taking the strains by weight percent: bacillus subtilis, yeast, lactic acid bacteria, photosynthetic bacteria, nitrifying bacteria, bacillus licheniformis, and the like. The preparation method comprises the following steps of: firstly, culturing the bacillus subtilis and bacillus licheniformis: culturing the bacillus subtilis and bacillus licheniformis on a beef extract peptone culture medium; secondly, culturing the yeast: culturing the yeast on a lactic acid-potato-glucose culture medium; thirdly, culturing the lactic acid bacteria: culturing the lactic acid bacteria on a wort culture medium, inoculating on a triangular flask for culturing, and finally inoculating on a solid culture medium for culturing; fourthly, culturing the photosynthetic bacteria; fifthly, culturing the nitrifying bacteria; and sixthly, stirring and mixing the cultured strains by weight ratio, and drying at low temperature, thereby obtaining the compound microorganism fungicide. The formula is reasonable; the use is convenient; water is uniformly splashed; the method is easily performed; and the operation is convenient.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and more specifically relates to a compound microbial preparation for improving the water quality of aquaculture water, and also relates to a preparation method of the biological preparation. Fish farming. Background technique [0002] With the development of the aquaculture industry, industrial high-density farming requires a large amount of feed, and the residue of bait and the excrement of aquatic animals dissolve in the water, causing pollution to the aquaculture water, resulting in the increase of organic matter, NH3, H2S and other harmful substances in the water. At the same time, the chemical oxygen demand (COD) and biological oxygen demand (BOD) remain high, and the above-mentioned negative effects can be effectively eliminated by using microbial agents. The application of microbial preparations in aquaculture improves the ecological environment of water bodies, inhibits and kills...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/16C12N1/20C02F3/34C12R1/125C12R1/225C12R1/10
Inventor 徐维烈杨涛袁庆丰杨亭
Owner 武汉合缘绿色生物股份有限公司
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