Effect testing method for duck hemorrhagic ovaritis inactivated vaccine

A technology of hemorrhagic oophoritis and inactivated vaccines, which is applied in the field of veterinary biological products inspection, to achieve the effect of objective method and strong operability

Active Publication Date: 2012-10-17
INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no detailed reports on objective, feasible, operable and scientific vaccine efficacy evaluation methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Duck Hemorrhagic Ovariitis Inactivated Vaccine Efficacy Testing Age in Days of Ducks

[0021] The Peking ducks aged 42, 50, 100, 120, 200, 300, 400, 560, and 600 days were divided into 9 groups with 10 birds in each group, and each group was Peking ducks of the same age (purchased from Beijing Jinxing Duck Industry Center Nankou breeding duck farm, duck hemorrhagic ovariitis antibody was negative). The test ducks were challenged with the same dose of DHOV-HB strain virus by oral route, containing about 3×10 4 ELD 50 / Only. Duck blood was collected through the wing vein 5 days after the challenge, and the serum was separated aseptically. Serum was inoculated with 6-day-old SPF chicken embryos via the yolk sac route, 5 eggs / duck, 0.2ml / embryo, and incubated at 37°C for 168 hours. Chicken embryos that died within 24 hours were discarded, and the number of chicken embryos that died within 24 to 168 hours and were positive for DHOV by RT-PCR was calculated. On...

Embodiment 2

[0025] Embodiment 2 Duck hemorrhagic ovariitis inactivated vaccine efficacy evaluation with the sex of the duck

[0026] Eighty 140-day-old Peking ducks of half male and half male were orally challenged with the same dose of DHOV-HB strain virus, containing about 3×10 4 ELD 50 / Only. From 1 to 8 days after the challenge, the blood of ducks was collected through the wing vein, 10 ducks per day, and the serum was separated aseptically. Serum was inoculated with 6-day-old SPF chicken embryos via the yolk sac route, 5 eggs / duck (each duck was inoculated with 5 chicken embryos), 0.2ml / embryo, and incubated at 37°C for 168 hours. Chicken embryos that died within 24 hours were discarded, and the number of chicken embryos that died within 24 to 168 hours and were positive for DHOV was calculated. One or more of the 5 chicken embryos in each serum was dead and DHOV positive was recorded as the duck virus isolation positive. The positive rate of virus isolation in female Peking duck...

Embodiment 3

[0027] Example 3 Immune protection-gross lesion method for duck hemorrhagic ovariitis inactivated vaccine efficacy evaluation (1)

[0028] Use the inactivated duck hemorrhagic ovariitis vaccine (batch number 2012001, developed by the Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences and Ruipu (Baoding) Biopharmaceutical Co., Ltd.) to immunize 10 200-day-old Peking ducks, half male and half male, as a vaccine immunization Group. The immunization dose and method of the vaccine immunization group are as follows: the first immunization vaccine dose is 0.5ml / bird, and the chest muscle is injected. Two weeks after the first immunization, follow the same dose and the same route for the second immunization, and 5 weeks after the second immunization, the challenged virus strain is DHOV-HB strain, and the intramuscular injection route is used to challenge the virus. The challenge dose for each duck is about 3×10 4 ELD 50 . At ...

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Abstract

The present invention provides an effect testing method for a duck hemorrhagic ovaritis inactivated vaccine. According to the method, a duck hemorrhagic ovaritis inactivated vaccine is adopted to vaccinate ducks twice, the second time vaccination is performed after the first time vaccination is performed two weeks, duck hemorrhagic ovaritis virus is adopted to carry out virus challenge on the vaccinated ducks after the second time vaccination is performed 3-5 weeks, the tested ducks are killed after the virus challenge is performed 5-10 days, autopsy is performed, and general pathological changes of a genital system are observed, wherein the pathological changes occur if the female ducks present ovarian follicle bleeding, deformation and oviduct atrophy, or the male ducks present testis atrophy and deferent duct atrophy, and the qualified standard of the vaccine effect test is that the virus challenge protection rate of the vaccinated ducks without pathological changes of the genital system is more than 70%.

Description

technical field [0001] The invention relates to a method for testing the efficacy of inactivated vaccines for veterinary use, in particular to a method for testing the efficacy of inactivated vaccines for duck hemorrhagic oophoritis, which belongs to the technical field of testing biological products for animals. Background technique [0002] Duck Hemorrhagic Ovaritis (DH) is a newly popular, acute, and highly contagious duck infectious disease. The pathogen is Duck Hemorrhagic Ovaritis Virus (DHOV). Classified as a member of the Flaviviridae, it infects ducks of different ages, causing ovarian hemorrhage, testicular atrophy, egg production, fertilization rate and hatching rate of sexually mature ducks, and increased duck death rate. [0003] In the study of the taxonomic status of the virus, through the sequence homology analysis and evolution analysis of the genome sequence of the F4 generation duck embryo passage virus of the isolated virus strain and known viruses, it wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15
Inventor 刘月焕杨保收韩春华何平有林健郁宏伟朱秀同刘浩毛雅园徐倩倩赵际成段会娟潘洁梁武
Owner INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI
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