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Method for promoting gonad maturing of pseudorasbora parva out of season

A technology of ear fish and gonads, which is applied in the field of aquatic animal reproduction, can solve the problem that the gonads of ear fish have not yet been seen, and achieves the effects of low equipment requirements, low cost and easy operation.

Inactive Publication Date: 2012-10-24
HUAZHONG AGRI UNIV
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Problems solved by technology

[0005] So far, there have been no related reports on promoting the maturation of the gonads of the earfish in anti-season

Method used

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  • Method for promoting gonad maturing of pseudorasbora parva out of season
  • Method for promoting gonad maturing of pseudorasbora parva out of season
  • Method for promoting gonad maturing of pseudorasbora parva out of season

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Embodiment Construction

[0021] The implementation and application of the present invention will be described below in conjunction with the embodiments.

[0022] Embodiments of the present invention are carried out in the Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, Fisheries College, Huazhong Agricultural University, and the specific implementation steps are as follows:

[0023] (1) Selection of broodstock: In October 2010, 500 cods weighing 0.8-2.5 g were obtained from Liangzi Lake, Hubei Province, and transported back to the laboratory with oxygen bags.

[0024] (2) Temporary support: After immersing the fish body in 2 to 3% salt water for 5 minutes, put it into a circular fiberglass tank (60 cm in diameter, 80 cm in height, and 180 L in water volume) of a circulating aquaculture system and temporarily support it for 2 weeks. During the temporary raising period, the feed is based on the daily ration, which is 5-8% of the fish body weight. The feed...

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Abstract

The invention belongs to the technical field of aquatic livestock reproduction, and relates to a method for promoting the gonad maturing of pseudorasbora parva out of season. The method comprises the following steps of: (1) in the middle and lower reaches of Changjiang River, selecting pseudorasbora parva longer than 40mm and heavier than 1.0g in September to October, and carrying the pseudorasbora parva back with an oxygen bag as a parent; (2) soaking the pseudorasbora parva body in 2-3% saline solution for 5 minutes, and temporarily cultivating for 2 weeks in a circulating aquaculture system, wherein the daily feed is 5-8% of the pseudorasbora parva weight in the temporary cultivation period; (3) transferring the pseudorasbora parva into an aquarium with a circulating filtration and oxygen increasing device, wherein the density is 12 pseudorasbora parvas per aquarium; and controlling the water temperature to 22-24 DEG C, the light intensity on water surface is 400lux, and the illumination time is 16-24h / d; (4) feeding the pseudorasbora parvas in the morning and afternoon everyday; and (5) changing water once every 2 weeks, wherein 1 / 2 of the cultivation water is changed; and the physicochemical conditions of water are: the dissolved oxygen is greater than or equal to 7.5mg / L, the pH is 7.5-8.0, the ammonia nitrogen is less than or equal to 0.1mg / L, and the nitrite nitrogen is less than or equal to 0.01mg / L; and cultivating for 2 months to obtain the pseudorasbora parva with mature gonad development.

Description

technical field [0001] The invention belongs to the technical field of aquatic animal reproduction, and in particular relates to a method for promoting the maturation of the gonads of the wheat ear fish against the season. Background technique [0002] Fish toxicity test materials are indispensable for formulating water quality benchmarks or standards for surface water and fishery waters of toxic chemicals, and for evaluating the impact of toxic chemicals on aquatic ecosystems. For a long time, my country has used economic fish such as green carp, grass carp, silver carp and bighead carp as acute toxicity test materials, but due to the long time of sexual maturity and the large size of the fish species, the test is subject to certain restrictions; the Organization for Economic Cooperation and Development also uses (OECO) and the U.S. Environmental Protection Agency (USEPA) recommended fish such as medaka, zebrafish, and black-headed minnow as toxicity test materials, but thes...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 祝东梅杨坤曾聪宋文王卫民
Owner HUAZHONG AGRI UNIV
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