Method for culturing special honeysuckle for desertification control
A cultivation method, honeysuckle technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of large investment in desert control, low efficiency, and inability to maintain circular development, etc., to achieve strong reproductive ability and renewal ability , developed root system, and strong stress resistance
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Embodiment 1
[0081] Material:
[0082] 1. Explant honeysuckle.
[0083] On March 4, 2003, an excellent single plant of 1-year-old traditional authentic Da Maohua was planted from Pingyi County Jingcheng Seedling Stock Co., Ltd.
[0084] 2. Media and medicines were purchased from Shandong Linyi Chemical Market.
[0085] 3. Tools
[0086] ⑴ dissecting mirror
[0087] ⑵Microscope
[0088] ⑶ culture vessel
[0089] ⑷ bracket balance
[0090] ⑸Electronic analytical balance
[0091] ⑹Pipette
[0092] ⑺Pipette gun
[0093] ⑻ Volumetric flask Measuring cylinder Beaker
[0094] ⑼ Light incubator
[0095] ⑽ Refrigerator
[0096] ⑾ Bottle rack
[0097] ⑿ Acidity Meter
[0098] ⒀ Knife, scissors, tweezers
[0099] ⒁ Micrometer
[0100] ⒂ Chromosome analysis system instrument
[0101] ⒃ Sterile workbench
[0102] ⒄ Autoclave
[0103] 4. Method
[0104] 1) Explant selection and disinfection treatment, select healthy plants with mature growth, pure varieties, good flower color, and no ...
Embodiment 2
[0162] 1. Materials
[0163] 1.1, explant honeysuckle.
[0164] On April 3, 2003, an excellent single plant of 1-year-old traditional authentic Da Maohua was planted from the Muzishan Honeysuckle Science and Technology Park in Zhengcheng Town.
[0165] 1.2. Culture medium and medicines were purchased from Shandong Linyi Chemical Market.
[0166] 1.3. Tools
[0167] ⑴ dissecting mirror
[0168] ⑵Microscope
[0169] ⑶ culture vessel
[0170] ⑷ bracket balance
[0171] ⑸Electronic analytical balance
[0172] ⑹Pipette
[0173] ⑺Pipette gun
[0174] ⑻ Volumetric flask Measuring cylinder Beaker
[0175] ⑼ Light incubator
[0176] ⑽ Refrigerator
[0177] ⑾ Bottle rack
[0178] ⑿ Acidity Meter
[0179] ⒀ Knife, scissors, tweezers
[0180] ⒁ Micrometer
[0181] ⒂ Chromosome analysis system instrument
[0182] ⒃ Sterile workbench
[0183] ⒄ Autoclave
[0184] 2. Method
[0185] 1) Explant selection and disinfection treatment, select healthy plants with mature growth, ...
Embodiment 3
[0230] Embodiment 3 The comparison of the effective drug content of the honeysuckle that the present invention cultivates and the traditional Da Maohua
[0231] The dried flowers produced by the new seedlings of honeysuckle cultured by the method of Example 1 of the present invention and the dried flowers of the parental explants are compared and tested by the [2000] edition Pharmacopoeia "High Performance Liquid Chromatography" used by the Institute of Medicinal Botany, Chinese Academy of Medical Sciences, and the results Such as figure 1 shown.
[0232] From figure 1 From the results of A, it can be seen that the two types of honeysuckle are at 3399, 2923, 2852, 1736, 1637 (1634), 1549, 1403, 1317, 1260, 1102, 1051, 764 (763) cm -1 There is a characteristic absorption peak at the wave number, and the peak position and peak shape of the main absorption peaks of the two kinds of honeysuckle are roughly the same, indicating that the chemical composition of the two is basicall...
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