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Eucalyptus PGEF12 gene as well as plant expression vector thereof, host cell thereof and application thereof

A plant expression vector and plant technology, applied in the direction of botany equipment and methods, applications, plant peptides, etc., can solve problems such as complex genetic diversity, and achieve the goals of increasing plant biomass, promoting plant vegetative growth, and promoting root growth Effect

Inactive Publication Date: 2013-08-28
SINO FOREST CHINA INVESTMENTS LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Forest trees and other woody plants are rich in genetic resources and complex genetic diversity, with many genes with potential application value but not yet fully explored and effectively isolated

Method used

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  • Eucalyptus PGEF12 gene as well as plant expression vector thereof, host cell thereof and application thereof
  • Eucalyptus PGEF12 gene as well as plant expression vector thereof, host cell thereof and application thereof
  • Eucalyptus PGEF12 gene as well as plant expression vector thereof, host cell thereof and application thereof

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Embodiment Construction

[0026] The present invention will be described in more detail below through specific embodiments and with reference to the accompanying drawings. It should be understood that the examples described below are only for illustration rather than limitation of the present invention.

[0027] 1. Cloning and sequence analysis of PGEF12 gene

[0028] 1. Preparation of Eucalyptus cDNA

[0029] Choose fresh eucalyptus ( Eucalyptus ) leaves, such as Eucalyptus eucalyptus species DH32-29 ( Eucalyptus urophylla × grandis cv DH32-29) leaves were quickly frozen in liquid nitrogen, and then stored in a -80°C ultra-low temperature freezer (SANYO, MDF-382E) for later use.

[0030] Total RNA was extracted from Eucalyptus leaves according to the existing Cetyltrimethyl Ammonium Bromide (CTAB) extraction method (Chang et al., Plant Molecular Biology Reporter 11(2): 113-116 (1993)). Extracted RNA was treated with RNase-free DNase (Takara, Cat# D2270A) to remove contaminating genomic DNA.

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Abstract

The invention provides an eucalyptus PGEF12 gene. A cDNA sequence of the eucalyptus PGEF12 gene is characterized in that the cDNA sequence has a DNA sequence shown by SEQ ID NO. 1 or a code of the eucalyptus PGEF12 gene has a polypeptide of an amino acid sequence shown by SEQ ID NO. 2. The invention further provides a plant expression vector, which comprises the eucalyptus PGEF12 gene. The invention further provides the above gene, the plant expression vector or an application of a host cell the gene including the plant expression vector in aspects of stimulating plant vegetative growth and increasing plant biomass; and the application is specifically as follows: the gene and / or the plant expression vector is transferred into a plant; or the plant is infected by the host cell. Through increasing an expression index of the PGEF12 in the plant, the number of leaves of the plant as well as the diameter of the plant can be increased, the growth of a root can be promoted and the biomass can be increased. The technical scheme provided by the invention can be used for increasing yields of forests, vegetables and commercial crops.

Description

technical field [0001] The invention relates to plant genes and applications thereof, in particular to eucalyptus PGEF12 gene and its plant expression vector and its application in promoting plant vegetative growth and increasing plant growth. Background technique [0002] Trees play an important role in air purification, windbreak and sand fixation, soil and water conservation, ecological balance and biodiversity, and provision of high-quality timber and high-yield and high-quality forest fruits. They have huge ecological benefits, social benefits and economic value. Forest trees and other woody plants are rich in gene resources and complex genetic diversity, with many genes with potential application value but not yet fully explored and effectively isolated. In recent years, with the development of molecular biology, molecular genetics, genomics and bioinformatics, and the establishment and improvement of library construction and screening technology, gene cloning technolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C12N1/21C12N5/10C07K14/415A01H5/00
Inventor 李奕恒孙长斌许文钊
Owner SINO FOREST CHINA INVESTMENTS LTD
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