Cordyceps sobolifera fruiting body with cicada larva as host and culture method thereof

A cultivation method and technology of cicadae, which is applied in the field of cicadae fruiting body and its cultivation, can solve the problems such as inability to obtain cicadae, and achieve the effect of safe and reliable consumption and high nutritional content

Inactive Publication Date: 2012-11-07
桐乡市中泰虫草专业合作社
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In addition, rice, wheat, etc. are used as the fruiting body of the cicadae produced by the culture medium, and there is no cicada body, so it is impossible to obtain the cicadae with the same shape as the natural one, and there is no relevant report on producing the cicadae with the cicada larvae

Method used

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  • Cordyceps sobolifera fruiting body with cicada larva as host and culture method thereof
  • Cordyceps sobolifera fruiting body with cicada larva as host and culture method thereof
  • Cordyceps sobolifera fruiting body with cicada larva as host and culture method thereof

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Embodiment

[0023] Step 1: Sampling: Take a sample of Cordyceps militaris (La.: Fr), cut 1-2cm long pieces at the front of the fruiting body of Cordyceps militaris and put them in a petri dish for cultivation;

[0024] The second step: strain separation: Cordyceps militaris sporocarps (provided by the Cordyceps research group of Shanghai Academy of Agricultural Sciences) are separated, purified and rejuvenated through spores on a solid medium to obtain Cordyceps militaris. The culture temperature is 20°C and the culture time is 12 days. Eliminate the mycelium with many fluffs and no color change, and select the mycelium that has good color change, fast color change, and grows fast against the culture medium;

[0025] The third step: the seed mycelium obtained in the second step is shaken and cultivated with liquid medium for 4-5 days, the culture temperature is 20°C-22°C, the rotation speed is 120r / m, and there are small bacterial balls filled with liquid medium That is, it can be made in...

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Abstract

The invention discloses a culture method of Cordyceps sobolifera fruiting body with cicada larva as a host. The culture method includes: subjecting Cordyceps militaris fruiting body to spore isolation, purification and rejuvenation on solid medium to obtain Cordyceps militaris mycelia, and selecting the mycelia which have good and fast coloring, are attached to the medium and grow well as reserved strains; subjecting the reserved mycelia to shaking culture with liquid medium to obtain a liquid strain; treating larvae of cicada, namely pricking body of each larva to make small holes with an aseptic needle, and using an aseptic syringe to suck in the liquid strain to inject the back of each larva; placing the injected and infected larvae in aseptic tissue culture bottles, using a syringe to inject 0.1ml of the liquid strain into an outer body of each larva, closing bottle covers, placing the bottles in the dark for moisture-preserved culture, placing the bottles in sunlight for 20 days, injecting aseptic water, and continuing culture till the Cordyceps sobolifera fruiting body grows to be 3cm long. Compared with fruiting body of natural Cordyceps sobolifera, the Cordyceps sobolifera fruiting body cultured by the method has matching nutrients and medicinal components, is high in quality, high in infection success rate, high in growth rate, short in time and low in cost.

Description

technical field [0001] The invention relates to a cicada flower fruiting body with cicada larvae as a host and a cultivation method thereof. Background technique [0002] Cicada flower (Cordyceps sobolifera; C.cicadae), also known as cicada chrysalis, is a wonderful biological complex with animal and plant characteristics. Its biological root is the larvae of cicada pupa or mountain cicada. -Grow out from the head of 3 cicada larvae, about one inch long, flowering and branching from the top. Cicadae belongs to the phenomenon of biological pathology, which is a combination of insects and fungi. When autumn comes, cicadas burrow into the soil and gradually turn into cicada pupae. They are parasitized by Cordyceps fungi before they emerge. The mycelium completely takes over and only a shell remains. When all things recover, the mycelium transforms from the vegetative stage to the sexual stage, and gradually blooms and branches from the top, so it is named cicadae. It is main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 徐正华沈金德徐窕沈娟丽
Owner 桐乡市中泰虫草专业合作社
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