Sample diluent of oral mucosal transudate for detecting HIV antibody by colloidal gold method, and preparation method thereof

Abstract

The invention discloses a sample diluent of oral mucosal transudate for detecting HIV antibody by colloidal gold method, and a preparation method thereof, belonging to the technical field of biological detection. The diluent comprises disodium hydrogen phosphate 17.3-19.2 g/L, sodium dihydrogen phosphate 2.6-2.9 g/L, sodium chloride 8.3-9.2 g/L, 10% tween 80 9.5-10.5 mL/L, serum 142.5-157.5 mL/L, streptavidin 28.5-31.5 mg/L, biotin 19-21 mL/L, EDTA-2Na 19-21 g/L, NaN3 2.9-3.2 mL/L, and the balance of purified water. The inventive sample diluent can accelerate reaction speed and enable detection to be smoother.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a sample diluent for detecting HIV antibody by the colloidal gold method of oral mucosa exudate and a preparation method thereof. Background technique [0002] Immunocolloidal gold technology is a simple, fast and accurate detection technology developed in the 1990s. Its principle is similar to double-sandwich ELISA. All reagents were immobilized on a microporous nitrocellulose membrane in advance. When using, place one end in the detection solution, and the nitrocellulose membrane can be passed through by the liquid, if the detection solution contains relevant antigens or antibodies. A light red line will appear in the detection area, which means it is positive, otherwise it is negative. Since the technology came out, it has been continuously adopted clinically. This method does not rely on sophisticated instruments, does not rely on skilled technicians, and has simple oper...

AI Technical Summary

Problems solved by technology

However, the current method of using oral mucosal exudate as a sample to detect AIDS antibodies is still in its infancy, and there has been no report on a sample diluent specially used to detect HIV antibodies in oral mucosal exudate.